摘要：【中文】本发明提供了一种基于宏基因组学的病原微生物检测方法及试剂盒，所述方法包括如下步骤：核酸提取；高通量测序文库构建；将高通量测序文库构建中构建的测序文库进行定量；将定量后的测序文库，稀释至1‑4pM，在测序仪上进行高通量测序；对高通量测序中获得的测序数据进行分析。本发明的另一方面，还提供了一种病原微生物检测试剂盒，本发明所提供的基于宏基因组学的高通量测序文库构建方法及试剂盒，使用片段化酶对DNA进行片段化，DNA的损失较机械法更小，同时片段化的速率也更快，提高了整个文库的构建效率。 【EN】The invention provides a pathogenic microorganism detection method and a kit based on metagenomics, wherein the method comprises the following steps: extracting nucleic acid; constructing a high-throughput sequencing library; quantifying the sequencing library constructed in the high-throughput sequencing library construction; diluting the quantitative sequencing library to 1-4pM, and performing high-throughput sequencing on a sequencer; sequencing data obtained in high-throughput sequencing was analyzed. According to the high-throughput sequencing library construction method and kit based on the metagenomics, provided by the invention, the DNA is fragmented by using the fragmenting enzyme, so that the loss of the DNA is smaller than that of a mechanical method, the fragmentation rate is higher, and the construction efficiency of the whole library is improved.