摘要：【中文】本发明公开了一类新型A型肉毒毒素轻链多肽类抑制剂及其制备方法与应用。所述多肽类抑制剂的结构式如式I所示。本发明通过Fmoc‑固相肽合成法得到线性肽，然后酰胺化关环，纯化后得到目标物。各抑制剂对A型肉毒毒素轻链抑制IC₅₀处于0.37～3.1μM，能显著延长A型肉毒毒素中毒小鼠的死亡时间。本发明对A型肉毒毒素的中毒治疗具有应用价值。 【EN】The invention discloses a novel A-type botulinum toxin light chain polypeptide inhibitor and a preparation method and application thereof. The structural formula of the polypeptide inhibitor is shown as a formula I. The invention obtains linear peptide by Fmoc-solid phase peptide synthesis, then amidates to close the ring, and obtains the target object after purification. Each inhibitor inhibits botulinum toxin type A light chain IC₅₀The death time of the mouse suffering from the botulinum toxin type A poisoning can be remarkably prolonged when the mouse is at 0.37-3.1 mu M. The invention has application value in the treatment of the botulism A.

摘要：【中文】本发明公开了一种筛选适宜机械化收获棉花品种的方法，该方法包括：S1、选择合适栽植土壤，选择排灌方便，地势平坦，田园化质量高，肥力水平高且均匀的苗床，在每平米的土壤中浇灌10升营养液；S2、将苗床建立常规黄萎病育种圃，收集黄萎病棉花的枯枝落叶和残体以及有机肥，经过酵制后，与土壤拌匀，对苗床进行均匀铺设，将处理后棉籽播种在苗床上，本方法采用培育棉种每代都在带有病菌体的土壤中生长进行，使培育棉种结果更加可靠，利用杂交、自交纯化遗传组合选育出新抗黄萎病棉花品种，同时也在抗黄萎病的过程中进行耐旱检测及抗虫检测，培育棉种提高了抗黄萎病发病率，使棉花性能稳定，产量提高，提高了棉花种植的经济效益。 【EN】The invention discloses a method for screening cotton varieties suitable for mechanized harvesting, which comprises the following steps: s1, selecting proper planting soil, selecting a seedbed which is convenient to drain and irrigate, flat in terrain, high in rural chemical quality, high in fertility level and uniform, and irrigating 10 liters of nutrient solution in each square meter of soil; s2, establishing a conventional verticillium wilt breeding garden on a seedbed, collecting the dry branches, fallen leaves, residues and organic fertilizers of the verticillium wilt cotton, fermenting, uniformly mixing with soil, uniformly paving the seedbed, and sowing the treated cotton seeds on the seedbed.