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[发明]
【中文】发酵生产腺苷的分离纯化方法 【EN】Separation and purification method for producing adenosine by fermentation
申请号:
201911426656.9
公开号:CN111171097A 主分类号:C07H19/167
申请人:
【中文】赤峰蒙广生物科技有限公司【EN】CHIFENG MENGGUANG BIOTECHNOLOGY Co.,Ltd.
申请日:2019.12.29 公开日:2020.05.19
发明人:
【中文】张莹
;
施明安【EN】Zhang Ying
;
Shi Mingan
摘要:【中文】本发明公开了一种发酵生产腺苷的分离纯化方法,该方法采用微滤分离技术去除发酵液中的菌体,再用超滤分离去除微滤液中的大分子蛋白等杂质,然后用大孔树脂吸附超滤液中的腺苷,并用酸洗脱,串联阴离子交换树脂去除杂质并中和pH,最后浓缩、脱色、结晶分离得到腺苷成品。本发明的提取纯化工艺除杂效果好,一次结晶就能够获得合成产品,成品腺苷液相纯度≥99.5%,外标含量≥99.5%,总收率≥80%,且不需要硅藻土等助滤剂过滤,无固废产生,安全环保,具有良好的经济效益。 【EN】The invention discloses a separation and purification method for producing adenosine by fermentation, which adopts microfiltration separation technology to remove thalli in fermentation liquor, then uses ultrafiltration separation to remove impurities such as macromolecular protein in micro-filtrate, then uses macroporous resin to adsorb adenosine in the ultra-filtrate, uses acid to elute, connects anion exchange resin in series to remove impurities and neutralize pH, and finally obtains adenosine finished products by concentration, decoloration, crystallization and separation. The extraction and purification process disclosed by the invention has a good impurity removal effect, a synthetic product can be obtained through one-time crystallization, the liquid phase purity of the finished adenosine is more than or equal to 99.5%, the external standard content is more than or equal to 99.5%, the total yield is more than or equal to 80%, and the filtration of filter aids such as diatomite and the like is not needed, so that no solid waste is generated, the process is safe and environment-friendly, and good economic benefits are achieved.
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2:
[发明]
【中文】电控外摆门结构及具有其的车辆 【EN】Electric control outward swinging door structure and vehicle with same
申请号:
201911285213.2
公开号:CN111005643A 主分类号:E05F15/41
申请人:
【中文】潍柴动力股份有限公司【EN】Weichai Power Co.,Ltd.
申请日:2019.12.13 公开日:2020.04.14
发明人:
【中文】郑庆波
;
文海艳
;
郑文静
;
李明安
;
罗青东
;
史桂红【EN】Zheng Qingbo
;
Wen Haiyan
;
Zheng Wenjing
;
Li Mingan
;
Luo Qingdong
;
Shi Guihong
摘要:【中文】本发明属于车辆设备技术领域,具体涉及一种电控外摆门结构及具有其的车辆。本发明中的电控外摆门结构包括外摆门、侧围总成和转臂,外摆门的内部的一端设有第一门锁,侧围总成与第一门锁相对应处设有第一锁扣,转臂的一端与侧围总成相连接,转臂的另一端与外摆门相连接,转臂能够沿转臂的一端做水平圆周运动,其中,第一门锁可以配合第一锁扣对外摆门和侧围总成进行锁止。通过使用本技术方案中的电控外摆门结构,增加了第一门锁和第一锁扣,提升了外摆门的安全性能,将原有的外摆门开闭时的提升式机械锁止结构改为外摆门水平方向的开闭,防止了外摆门的下沉问题,提升了稳定性。 【EN】The invention belongs to the technical field of vehicle equipment, and particularly relates to an electric control outward swinging door structure and a vehicle with the same. The electric control outward swinging door structure comprises an outward swinging door, a side wall assembly and a rotating arm, wherein a first door lock is arranged at one end inside the outward swinging door, a first lock catch is arranged at a position, corresponding to the first door lock, of the side wall assembly, one end of the rotating arm is connected with the side wall assembly, the other end of the rotating arm is connected with the outward swinging door, and the rotating arm can do horizontal circular motion along one end of the rotating arm, wherein the first door lock can be matched with the first lock catch to lock the outward swinging door and the side wall assembly. Through using the automatically controlled outer swing door structure among this technical scheme, increased first lock and first hasp, promoted the security performance of outer swing door, the promotion formula machinery locking structure when opening and closing original outer swing door changes the switching of outer swing door horizontal direction into, has prevented the problem of sinking of outer swing door, has promoted stability.
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