摘要：【中文】本发明涉及了一种基于CRISPR/Cas9变换水稻果皮色(白至红、红至白)的基因编辑方法，其包括如下步骤：(1)合成转录因子rc基因的特定gRNA‑spacer；(2)构建CRISPR/Cas‑gRNA‑spacer载体；(3)采用农杆菌转化法转化水稻品种的成熟胚、再生植株。T₀代种子即可实现水稻品种果皮色的白至红、红至白的相互转变。红米在世界各国长期用作滋补食品，是水稻育种的重要目标之一。经过人工长期驯化的水稻果皮色为白色，抗性弱，而来自栽培稻脱驯化的杂草稻果皮色为红色，抗性强。通过相互转换果皮色，可能培育出抗性强的白\红果皮水稻品种。本技术具有良好的商业化应用前景。 【EN】The invention relates to a gene editing method for transforming the peel color (white to red and red to white) of rice based on CRISPR/Cas9, which comprises the following steps: (1) synthesizing a specific gRNA-spacer of a transcription factor rc gene; (2) constructing a CRISPR/Cas-gRNA-spacer vector; (3) mature embryos and regenerated plants of rice varieties are transformed by an agrobacterium transformation method. T is₀The mutual transformation of the white to red and red to white of the peel color of the rice variety can be realized by the generation of seeds. Red rice is used as a tonic food in countries of the world for a long time and is one of the important targets of rice breeding. Long-term domesticated riceThe peel color of the weedy rice is white and the resistance is weak, while the peel color of the weedy rice which is removed and domesticated from the cultivated rice is red and the resistance is strong. By mutually converting the color of the peel, the rice variety with the white/red peel and strong resistance can be cultivated. The technology has good commercial application prospect.

摘要：【中文】本发明属于环境保护技术领域，涉及一种生物防除加拿大一枝黄花的方法，通过采用液体生产菌种和运输途中固体发酵的定制式大批量生产生物除草剂颗粒，改进的固体基质配方，双链条旋耕播撒一体机翻耕暴露加拿大一枝黄花地下根茎和撒播生物除草剂颗粒同步完成，使生物除草剂颗粒与加拿大一枝黄花地下根茎充分接触，并能够充分利用地下土壤的水分迅速萌发侵染，显著提高防效，实现一次处理完全根除加拿大一枝黄花的效果。具有高效、省工、省力、环保、安全、彻底的特点。 【EN】The invention belongs to the technical field of environmental protection, and relates to a method for biologically preventing and removing solidago canadensis, which comprises the steps of producing biological herbicide particles in batches by adopting liquid production strains and solid fermentation in the transportation process in a customized mode, improving the formula of a solid matrix, and synchronously completing the plowing and exposing the underground roots and stems of solidago canadensis and the sowing of the biological herbicide particles by a double-chain rotary tillage and sowing integrated machine, so that the biological herbicide particles are fully contacted with the underground roots and stems of solidago canadensis, the moisture in underground soil can be fully utilized to rapidly germinate and infect the solidago canadensis, the control effect is remarkably improved, and the effect of completely eradicating solidago canadensis by one-time treatment is realized. Has the characteristics of high efficiency, labor saving, environmental protection, safety and thoroughness.

摘要：【中文】本发明公开了一种利用大气偏振E‑矢量模式特征点的三轴姿态确定方法。该方法是采用如下步骤实现的：（1）利用复眼相机在线实时采集大气偏振模式；（2）将所采集的大气偏振模式作为三维定姿模型的原始数据，利用聚类分析求出太阳投影点及光轴方向矢量；（3）利用奇异值分解，确定矢量对转换中的姿态变换矩阵，进一步确定出三轴姿态。本发明设计合理，采用大气偏振E‑矢量模式进行三维定姿，提供了一种很好的技术手段，具有完全自主特性，通过对大气偏振模式分布的检测和处理，可以解算出载体的三轴姿态，简单可行且没有累积误差，同时偏振信息存在于大气层内的可见光中，很难被人为的大面积干扰和破坏。 【EN】The invention discloses a method for determining three-axis attitude by utilizing atmospheric polarization E-vector mode characteristic points. The method is realized by adopting the following steps: (1) collecting an atmospheric polarization mode on line in real time by using a compound eye camera; (2) the collected atmospheric polarization mode is used as the original data of the three-dimensional attitude determination model, and the sun projection point and the optical axis direction vector are solved by utilizing cluster analysis; (3) and determining an attitude transformation matrix in vector pair transformation by using singular value decomposition, and further determining the three-axis attitude. The invention has reasonable design, adopts the atmospheric polarization E-vector mode to carry out three-dimensional attitude determination, provides a good technical means, has completely autonomous characteristic, can solve the three-axis attitude of the carrier by detecting and processing the distribution of the atmospheric polarization mode, is simple and feasible, has no accumulated error, and has difficult artificial large-area interference and damage because the polarization information exists in the visible light in the atmosphere.

摘要：【中文】本发明属于检测装置技术领域，特别涉及管材对接检漏设备。其包括主夹具一和主夹具二，主夹具一和主夹具二底面的一侧设置下垫块，主夹具一和主夹具二上均设置密封胶垫通过口，密封胶垫环绕于待检测管材的对接处且两端置于密封胶垫通过口内，密封胶垫通过口的两端设置齿条通过口，两根齿条分别置于齿条通过口内，主夹具二表面设置电机，电机驱动齿条移动，主夹具一和主夹具二的外侧均设置锁紧装置，承插体一端置于密封胶垫与待检测管材之间，承插体的另一端连接真空表和真空泵。本发明结构简单，操作容易，通过观察真空表指针或数值的变动即可直观判断是否连接处存在泄漏，尤其适用于大尺寸管材的连接安装施工。 【EN】The invention belongs to the technical field of detection devices, and particularly relates to a pipe butt joint leakage detection device. It includes main anchor clamps one and main anchor clamps two, one side of main anchor clamps one and two bottom surfaces of main anchor clamps sets up down the cushion, all set up sealed cushion through-hole on main anchor clamps one and the main anchor clamps two, sealed cushion encircles and arranges sealed cushion through-hole in waiting to detect the butt joint department and both ends of tubular product in, the both ends of sealed cushion through-hole set up the rack through-hole, the rack through-hole is arranged respectively in to two racks, two surfaces of main anchor clamps set up the motor, motor drive rack removes, the outside of main anchor clamps one and main anchor clamps two all sets up locking device, sealed cushion is arranged in to socket joint body one end and is waited to detect between the tubular product, vacuum table and vacuum pump are connected to the other end of socket body. The invention has simple structure and easy operation, can visually judge whether the connection part has leakage or not by observing the pointer of the vacuum gauge or the change of the numerical value, and is particularly suitable for the connection installation construction of large-size pipes.

摘要：【中文】本发明涉及一种抗菌性自修复水凝胶及其制备方法。通过基于疏水作用的物理交联方法赋予凝胶自修复功能：通过引入胆碱‑氨基酸离子液体(VILs)赋予凝胶抗菌性和导电性：通过具有良好生物相容性的细菌纤维素(BC)增强其拉伸性能和韧性。制备该凝胶包含如下步骤：(1)胆碱‑氨基酸离子液体的合成；(2)乙烯基胆碱‑氨基酸离子液体单体(VILs)的制备；(3)细菌纤维素的纯化与水解；(4)聚胆碱‑氨基酸离子液体自修复凝胶的成型。本发明的凝胶自愈合效率高，具有良好的抗菌性、力学性能、导电性能及生物相容性，适用于生物、医疗、组织工程领域。 【EN】The invention relates to an antibacterial self-repairing hydrogel and a preparation method thereof. The gel is endowed with a self-repairing function through a physical crosslinking method based on hydrophobic effect: the gel is endowed with antibacterial property and conductivity by introducing choline-amino acid ionic liquids (VILs): the tensile property and the toughness of the Bacterial Cellulose (BC) are enhanced by the bacterial cellulose with good biocompatibility. The preparation of the gel comprises the following steps: (1) synthesizing choline-amino acid ionic liquid; (2) preparing vinyl choline-amino acid ionic liquid monomers (VILs); (3) purifying and hydrolyzing bacterial cellulose; (4) and forming the self-repairing gel of the choline-amino acid ionic liquid. The gel provided by the invention has high self-healing efficiency, good antibacterial property, mechanical property, conductivity and biocompatibility, and is suitable for the fields of biology, medical treatment and tissue engineering.

摘要：【中文】本发明涉及一种细菌纤维素/聚离子液体(BC/PILs)抗菌膜及其制备方法。其制备方法如下：(1)细菌纤维素的制备：采用静态培养法，以本实验室所筛选生产纤维素菌Komagataeibacter rhaeticus TJPU03制备细菌纤维素凝胶膜。(2)离子液体(ILs)单体的制备：选用天然化合物氨基酸、胆碱，制备新型绿色离子液体；在N‑羟基琥珀酰亚胺(NHS)和1‑乙基‑3‑(3‑二甲胺丙基)碳二亚胺(EDC)作用下，通过丙烯酸(AAc)将双键引入离子液体(ILs)，获得可聚合离子液体单体。(3)细菌纤维素/聚离子液体(BC/PILs)抗菌膜的制备：采用原位自由基聚合，将离子液体单体引入细菌纤维素网络，制备BC/PILs复合膜。该方法简单快捷，条件温和，不需要大型设备，水相反应绿色环保，所得复合膜具有良好的力学性能、生物相容性、生物降解性，药物缓释功能，抗菌性能，在生物医药、组织工程等领域存在广阔应用前景。 【EN】The invention relates to a bacterial cellulose/polyion liquid (BC/PILs) antibacterial film and a preparation method thereof. The preparation method comprises the following steps: (1) preparing bacterial cellulose: the bacterial cellulose gel membrane is prepared by adopting a static culture method and using the cellulose bacteria Komagataeibacter rhaeticus TJPU03 screened and produced in the laboratory. (2) Preparation of Ionic Liquid (ILs) monomers: selecting natural compounds of amino acid and choline to prepare novel green ionic liquid; introducing double bonds into Ionic Liquids (ILs) through acrylic acid (AAc) under the action of N-hydroxysuccinimide (NHS) and 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide (EDC) to obtain polymerizable ionic liquid monomers. (3) Preparing a bacterial cellulose/polyion liquid (BC/PILs) antibacterial film: and introducing an ionic liquid monomer into a bacterial cellulose network by adopting in-situ free radical polymerization to prepare the BC/PILs composite membrane. The method is simple and rapid, mild in condition, free of large-scale equipment, green and environment-friendly in aqueous phase reaction, and the obtained composite membrane has good mechanical property, biocompatibility, biodegradability, drug slow-release function and antibacterial property, and has wide application prospects in the fields of biomedicine, tissue engineering and the like.

摘要：【中文】本发明提供一种基于神经网络的文本分类方法，包括：将原始分类对象集中的各待分类对象分别输入至神经网络分类模型，以进行分类处理，得到原始分类对象集中的各待分类对象的分类结果，分类结果包括分类归属概率；筛选出分类归属概率小于预设概率阈值的待分类对象，以构成更新分类对象集；以及，更新神经网络分类模型，以得到新神经网络分类模型，新神经网络分类模型的深度不等于更新前的神经网络分类模型的深度；将更新分类对象集中的各待分类对象分别输入至新神经网络分类模型，以进行分类处理，得到更新待分类对象集中的各待分类对象的分类结果。通过对原始分类对象集进行多次分类处理，以提高待分类对象个体的分类正确率。 【EN】The invention provides a text classification method based on a neural network, which comprises the following steps: respectively inputting each object to be classified in the original classified object set into a neural network classification model for classification processing to obtain a classification result of each object to be classified in the original classified object set, wherein the classification result comprises a classification attribution probability; screening out objects to be classified with classification attribution probability smaller than a preset probability threshold value to form an updated classification object set; updating the neural network classification model to obtain a new neural network classification model, wherein the depth of the new neural network classification model is not equal to the depth of the neural network classification model before updating; and respectively inputting each object to be classified in the updated classified object set to the new neural network classification model for classification processing to obtain a classification result of each object to be classified in the updated classified object set. The classification accuracy of the individual objects to be classified is improved by carrying out multiple classification processing on the original classification object set.

摘要：【中文】本发明公开了一种测试多孔材料孔隙率的简易方法，该方法包括：裁取大小适量的薄片试样，测量所述试样的厚度为δ，在试样的一侧贴上防水的透明胶布，将贴有胶布的一侧朝下将试样置于水平桌面上，用胶头滴管滴一滴质量为m，体积为V(＝m/ρ，ρ为水的密度)的纯净水于试样未贴防水胶布的一面上，同时利用显微镜‑CCD‑电脑系统观测水在试样表面的渗流过程，经过足够长时间，水滴完全渗透到薄片多孔材料试样中时，拍摄得到水在试样中的半径为R的圆形渗流区域照片。渗流时间足够长，体积为V的水充满了整个渗流区域的孔隙，根据公式：计算所述试样的孔隙率η。本发明所提供的孔隙率测试方法，测试过程简单、易行、方便。此外，测试过程中所用仪器及材料的消耗方面成本低，性价比高，实用性强。 【EN】The invention discloses a simple method for testing porosity of a porous material, which comprises the following steps: cutting a sheet sample with a proper size, measuring the thickness of the sample to be delta, attaching a waterproof transparent adhesive tape on one side of the sample, placing the sample on a horizontal desktop with the side attached with the adhesive tape facing downwards, dropping a drop of pure water with the mass of m and the volume of V (m/rho, rho is the density of water) on the side of the sample which is not attached with the waterproof adhesive tape by using a rubber head dropper, observing the seepage process of water on the surface of the sample by using a microscope-CCD-computer system, and shooting to obtain a circular seepage area picture with the radius of R of the water in the sample when the water drops completely permeate into the sheet porous material sample after a sufficient time. The percolation time is long enough and the volume of water V fills the pores of the whole percolation region, according to the formula:the porosity of the sample is calculated η. the porosity testing method provided by the invention has simple and feasible testing process,Is convenient. In addition, the consumption of instruments and materials used in the test process is low in cost, high in cost performance and strong in practicability.

摘要：【中文】一种大规模糯性小麦籽粒鉴定的统筹方法，小麦籽粒进行横切，切面远离具胚端；具胚端的横切面进行碘‑碘化钾溶液轻染色，并将染色后的浮于具胚端表面的溶液吸干，再进行烘干；烘干后的所述具胚端通过专业人员进行颜色鉴定，保留糯性籽粒，将染色的所述具胚端直接播种并进行后期的农艺性状调查。本发明所述的大规模糯性小麦籽粒鉴定的统筹方法，大大节省了小麦籽粒的保存空间，而且避免了将小麦籽粒对照混乱造成失误；而且更有利于颜色的显示，减少了显色误差；烘干处理可以使颜色鉴别时更为方便，更便于所述具胚端的保存，减少保存过程中造成的籽粒发芽或籽粒腐烂的状况发生，从而大大提高了糯性小麦鉴定的效率。 【EN】A comprehensive method for large-scale waxy wheat grain identification comprises transversely cutting wheat grains, wherein the cut surface is far away from an embryo end; lightly dyeing the cross section with the embryo end with iodine-potassium iodide solution, sucking the dyed solution floating on the surface with the embryo end, and drying; and (3) carrying out color identification on the dried embryo-bearing end by a professional, reserving waxy grains, directly sowing the dyed embryo-bearing end, and carrying out later-stage agronomic character investigation. The overall method for identifying the large-scale waxy wheat grains greatly saves the storage space of the wheat grains and avoids errors caused by disordered contrast of the wheat grains; moreover, the color display is facilitated, and the color rendering error is reduced; the drying treatment can make the color identification more convenient, the preservation of the germ end is more convenient, and the seed germination or seed decay condition caused in the preservation process is reduced, thereby greatly improving the efficiency of the waxy wheat identification.

摘要：【中文】本公开提供了一种高频通信信道探测的方法和装置，属于通信技术领域。所述方法包括：接收多个探测时刻，所述多个探测时刻包括一年内多天中的多个时刻；若当前时刻在所述探测时刻之后的设定时隙内，探测并记录通信信道在多个频率上的通信质量；根据通信信道在各个所述探测时刻和各个所述频率上的通信质量，选择频率发射电波。本公开通过接收包括一年内多天中的多个时刻的探测时刻，若当前时刻在探测时刻之后的设定时隙内，探测并记录通信信道在多个频率上的通信质量，可以了解通信信道随经纬度、季节、日出日落等因素变化的情况，再根据通信信道在各个探测时刻和各个频率上的通信质量，选择频率发射电波，可以有效保证通信效果，避免通信中断。 【EN】The disclosure provides a method and a device for detecting a high-frequency communication channel, and belongs to the technical field of communication. The method comprises the following steps: receiving a plurality of probe instants, the plurality of probe instants comprising a plurality of instants in a plurality of days of a year; if the current time is in a set time slot after the detection time, detecting and recording the communication quality of a communication channel on a plurality of frequencies; and selecting the frequency to transmit the electric wave according to the communication quality of the communication channel at each detection time and each frequency. The method can know the condition of the communication channel changing along with factors such as longitude, latitude, season, sunrise, sunset and the like by receiving the detection time comprising a plurality of times in a plurality of days in one year, detecting and recording the communication quality of the communication channel on a plurality of frequencies if the current time is in the set time slot after the detection time, and then selecting the frequency to transmit electric waves according to the communication quality of the communication channel on each detection time and each frequency, thereby effectively ensuring the communication effect and avoiding communication interruption.