摘要：【中文】本发明提供了一种蛋白质提取方法和应用，具体涉及一种从石蜡包埋组织中提取蛋白质的方法和应用。本发明采用无毒的TO透明剂替换了有毒的二甲苯，安全环保；利用高温煮沸法对蛋白质进行提取处理，避免了常规操作的组织冻融裂解或超声破碎所致的低丰度蛋白质降解丢失；采用金属浴加热置换抗原修复液加微波加热的抗原修复步骤，配合嵌套筛网小室的使用，使得操作简单易行，并可较好地保证组织切片的完整性。本发明既提高了石蜡组织总蛋白质的提取量，又增加了对低丰度蛋白质的检测灵敏性；整体操作流程简单易行省时，安全无毒价廉。 【EN】The invention provides a protein extraction method and application, and particularly relates to a method for extracting protein from paraffin-embedded tissues and application. The invention adopts the nontoxic TO transparent agent TO replace the toxic dimethylbenzene, thereby being safe and environment-friendly; the protein is extracted by using a high-temperature boiling method, so that the degradation loss of low-abundance protein caused by freeze thawing and cracking or ultrasonic crushing of tissues in conventional operation is avoided; the antigen retrieval step of replacing the antigen retrieval liquid by metal bath heating and microwave heating is adopted, and the use of the nested screen chamber is matched, so that the operation is simple and easy, and the integrity of the tissue slice can be better ensured. The method improves the extraction amount of the total protein of the paraffin tissue, and increases the detection sensitivity of low-abundance protein; the whole operation flow is simple, easy and time-saving, safe, nontoxic and cheap.

摘要：【中文】本发明涉及一种结直肠癌标志物及其应用，具体涉及一种结直肠癌标志物TAF1L基因的异常表达及其检测方法和应用，所述结直肠癌标志物包括TAF1L基因异常表达的核酸和/或蛋白质，并通过运用基因测序技术、芯片半定量检测技术、qPCR定量检测技术、蛋白印迹半定量检测技术、免疫组织化学染色技术、原位杂交技术和液体活检技术能够实现TAF1L基因异常、核酸或/和蛋白质表达的检测，此检测的准确性较好、特异性较强、敏感性较高。 【EN】The invention relates to a colorectal cancer marker and application thereof, in particular to abnormal expression of a colorectal cancer marker TAF1L gene and a detection method and application thereof, wherein the colorectal cancer marker comprises nucleic acid and/or protein abnormally expressed by the TAF1L gene, and the detection of the TAF1L gene abnormality and the expression of the nucleic acid or/and the protein can be realized by applying a gene sequencing technology, a chip semi-quantitative detection technology, a qPCR quantitative detection technology, a western blot semi-quantitative detection technology, an immunohistochemical staining technology, an in-situ hybridization technology and a liquid biopsy technology, so that the detection has the advantages of better accuracy, stronger specificity and higher sensitivity.

摘要：【中文】本发明涉及一种结直肠癌标志物及其应用，具体涉及一种结直肠癌标志物GTF2H5基因的异常表达及其检测方法和应用，所述结直肠癌标志物包括GTF2H5基因异常表达的核酸或/和蛋白质，并通过运用基因测序技术、芯片半定量检测技术、qPCR定量检测技术、蛋白印迹半定量检测技术、免疫组织化学染色技术、原位杂交技术和液体活检技术能够实现GTF2H5基因异常、核酸或/和蛋白质表达的检测，此检测的准确性较好、特异性较强、敏感性较高。 【EN】The invention relates to a colorectal cancer marker and application thereof, in particular to abnormal expression of a colorectal cancer marker GTF2H5 gene and a detection method and application thereof, wherein the colorectal cancer marker comprises nucleic acid or/and protein abnormally expressed by the GTF2H5 gene, and the detection of the gene abnormality and the nucleic acid or/and protein expression of GTF2H5 can be realized by applying a gene sequencing technology, a chip semi-quantitative detection technology, a qPCR quantitative detection technology, a western blot semi-quantitative detection technology, an immunohistochemical staining technology, an in-situ hybridization technology and a liquid biopsy technology, so that the detection has the advantages of better accuracy, stronger specificity and higher sensitivity.