摘要：【中文】本发明属于基因诊断领域，公开了一种检测CYP1A2基因rs762551位点的实时荧光PCR方法及其引物探针组合。其中：上游引物FpG:5’‑GGTGAGCTCTGTGGGGC‑3’，上游引物FpG:5’‑GGTGAGCTCTGTGGGGA‑3’，下游引物Rp：5’‑GCTGAGGGTTGAGATGGAGAC‑3’，探针Probe：5’‑Cy5‑ACGCATGGTAGATGGAGCTTAG‑BHQ2‑3’。PCR反应完成后，可通过扩增曲线的有无并结合Ct值分析结果。采用本发明所提供的检测方法进行rs762551位点多态性的检测，具有操作简便、分辨率高、无污染、高通量等优点。 【EN】The invention belongs to the field of gene diagnosis, and discloses a real-time fluorescent PCR method for detecting rs762551 locus of CYP1A2 gene and a primer probe combination thereof. Wherein: an upstream primer FpG: 5'-GGTGAGCTCTGTGGGGC-3', an upstream primer FpG: 5'-GGTGAGCTCTGTGGGGA-3', a downstream primer Rp: 5'-GCTGAGGGTTGAGATGGAGAC-3', Probe Probe: 5 '-Cy 5-ACGCATGGTAGATGGAGCTTAG-BHQ 2-3'. After the PCR reaction is completed, the result can be analyzed by the presence or absence of an amplification curve and the combination of Ct values. The method for detecting the rs762551 site polymorphism has the advantages of simple and convenient operation, high resolution, no pollution, high flux and the like.

摘要：【中文】本发明提供了一种高纯相亚氧化钛的可控合成方法，高纯相亚氧化钛包括高纯相Ti₃O₅和高纯相Ti₄O₇，以偏钛酸为前驱体，将偏钛酸前驱体与还原剂置于同一密闭的反应器中煅烧，且所述偏钛酸前驱体与所述还原剂之间设有距离，向反应器内通入氩气，先于第一温度700～800℃下保温2～4h，再于第二温度800～1050℃下保温1～10h，即得到高纯相亚氧化钛。本发明采用前驱体与还原剂不接触的方法进行还原，通过偏钛酸在高温条件下钛氧键会断裂，生成高纯相亚氧化钛，同时会有氧和水蒸气溢出，水蒸气和氧在气流的作用下，会和旁边的还原剂发生反应，从而避免了因前驱体与还原剂混合反应，而出现产物与还原剂无法有效分离的问题。 【EN】The invention provides a controllable synthesis method of high-purity-phase titanium suboxide, and the high-purity-phase titanium suboxide comprises high-purity-phase Ti₃O₅And high purity phase Ti₄O₇The method comprises the steps of taking metatitanic acid as a precursor, placing the metatitanic acid precursor and a reducing agent in the same closed reactor for calcination, wherein a distance is reserved between the metatitanic acid precursor and the reducing agent, introducing argon into the reactor, preserving heat for 2-4 hours at a first temperature of 700-800 ℃, and preserving heat for 1-10 hours at a second temperature of 800-1050 ℃ to obtain the high-purity phase titanium suboxide. The method adopts a method that the precursor is not contacted with the reducing agent for reduction, titanium oxide bonds can be broken under the high-temperature condition through metatitanic acid to generate high-purity phase titanium suboxide, oxygen and water vapor can overflow simultaneously, and the water vapor and the oxygen can react with the reducing agent nearby under the action of airflow, so that the problem that the product and the reducing agent cannot be effectively separated due to the mixed reaction of the precursor and the reducing agent is solved.

摘要：【中文】本发明提供了一种惰性气氛下一步法制备高纯相Ti₄O₇纳米材料的方法，以偏钛酸为前驱体，将偏钛酸前驱体与还原剂碳粉混合后置于密闭的反应器中煅烧，向反应器内通入惰性气体，先于第一温度800～950℃下保温一定时间，再于第二温度950～1300℃下保温一定时间，即可得到高纯相Ti₄O₇。本发明采用偏钛酸为前驱体，以碳粉为还原剂，在反应后碳粉会转化成CO或CO₂溢出，而自动消除，无需进行后续的分离步骤，可实现一步法制备，大大简化了制备步骤，提高了产物的纯度。 【EN】The invention provides a method for preparing high-purity phase Ti by using an inert atmosphere through a one-step method₄O₇The method for preparing the nano material comprises the steps of taking metatitanic acid as a precursor, mixing the metatitanic acid precursor with reducing agent carbon powder, then placing the mixture into a closed reactor for calcination, introducing inert gas into the reactor, preserving heat for a certain time at a first temperature of 800-950 ℃, preserving heat for a certain time at a second temperature of 950-1300 ℃, and obtaining high-purity phase Ti₄O₇. The invention adopts metatitanic acid as a precursor and carbon powder as a reducing agent, and the carbon powder can be converted into CO or CO after reaction₂Overflow is automatically eliminated, subsequent separation steps are not needed, one-step preparation can be realized, the preparation steps are greatly simplified, and the purity of the product is improved.

摘要：【中文】本发明属于基因诊断领域，公开了一种检测ANKK1基因rs1800497位点的实时荧光PCR方法及其引物探针组合。其中：上游引物FpC:5’‑AGCTGGGCGCCTGCCGC‑3’，上游引物FpT:5’‑AGCTGGGCGCCTGCCGT‑3’，下游引物Rp：5’‑GCAAATGTCCACGCCCGCA‑3’，探针Probe：5’‑FAM‑AGCACTTTGAGGATGGCTGTGT‑BHQ2‑3’。PCR反应完成后，可通过扩增曲线的有无并结合Ct值分析结果。本发明采用本发明所提供的检测方法进行rs1800497位点多态性的检测，具有操作简便、分辨率高、无污染、高通量等优点。 【EN】The invention belongs to the field of gene diagnosis, and discloses a real-time fluorescence PCR method for detecting rs1800497 locus of ANKK1 gene and a primer probe combination thereof. Wherein: an upstream primer FpC: 5'-AGCTGGGCGCCTGCCGC-3', an upstream primer FpT: 5'-AGCTGGGCGCCTGCCGT-3', a downstream primer Rp: 5'-GCAAATGTCCACGCCCGCA-3', Probe Probe: 5 '-FAM-AGCACTTTGAGGATGGCTGTGT-BHQ 2-3'. After the PCR reaction is completed, the result can be analyzed by the presence or absence of an amplification curve and the combination of Ct values. The method for detecting the rs1800497 site polymorphism has the advantages of simplicity and convenience in operation, high resolution, no pollution, high flux and the like.

摘要：【中文】一种红米水酒的酿造方法，属于酿酒技术领域。本发明将红米淘洗干净后浸泡，沥干后蒸煮，随后加入酒曲，放入恒温培养箱中糖化发酵；加入水，搅拌均匀，密封后发酵；将所得酒糟过滤后离心，取上层清液即得红米水酒。本发明方法制备所得红米水酒，可以规范化生产佤族水酒，味道醇厚，入口甘美，回味绵长且营养价值高。 【EN】A brewing method of red rice water wine belongs to the technical field of wine brewing. Washing red rice, soaking, draining, cooking, adding distiller's yeast, and saccharifying and fermenting in a constant-temperature incubator; adding water, stirring, sealing, and fermenting; filtering the obtained distiller's grains, centrifuging, and collecting supernatant to obtain red rice wine. The red rice water wine prepared by the method can be used for producing Va nationality water wine in a standardized way, and has mellow taste, sweet and delicious taste, lasting aftertaste and high nutritional value.

摘要：【中文】本申请公开了一种应用于电动车的充电管理方法、装置和设备，涉及电动车技术领域。上述充电管理方法包括：建立电动车与移动终端之间的通讯连接，周期性采集上述电动车的外电路电压；每隔第一时长计算一次有效电压，其中，上述有效电压为当次第一时长内采集到的所有外电路电压的平均值；基于计算得到的至少两个有效电压，判断上述电动车是否处于正常充电状态；当上述电动车处于正常充电状态时，向上述移动终端输出第一指示信息，上述第一指示信息指示上述电动车处于正常充电状态。上述方案有利于用户及时获知电动车的实际充电情况。 【EN】The application discloses a charging management method, a charging management device and charging management equipment applied to an electric vehicle, and relates to the technical field of electric vehicles. The charging management method comprises the following steps: establishing communication connection between the electric vehicle and the mobile terminal, and periodically collecting the external circuit voltage of the electric vehicle; calculating effective voltage every other first time length, wherein the effective voltage is the average value of all the external circuit voltages collected in the first time length; judging whether the electric vehicle is in a normal charging state or not based on at least two effective voltages obtained by calculation; and when the electric vehicle is in a normal charging state, outputting first indication information to the mobile terminal, wherein the first indication information indicates that the electric vehicle is in the normal charging state. The scheme is favorable for a user to timely know the actual charging condition of the electric vehicle.

摘要：【中文】本发明公开一种车辆设防方法、系统及存储介质，该车辆设防方法应用于车辆设防系统，所述系统包括用户终端、车辆控制终端，所述用户终端与所述车辆控制终端通过蓝牙通信连接，该方法包括：在所述车辆控制终端检测到车辆熄火后，所述车辆控制终端检测与当前用户终端之间的蓝牙连接状态；在所述车辆控制终端检测到所述车辆控制终端与当前用户终端的蓝牙连接状态异常时，获取所述车辆的熄火时长；当所述熄火时长达到预设阈值时，所述车辆控制终端控制所述车辆进入设防模式。本发明实现了在当前用户终端与车辆控制终端即使蓝牙连接中断，或者连接信号较差时也能对车辆进行设防，从而提升了车辆安全性。 【EN】The invention discloses a vehicle fortification method, a system and a storage medium, wherein the vehicle fortification method is applied to a vehicle fortification system, the system comprises a user terminal and a vehicle control terminal, the user terminal is in communication connection with the vehicle control terminal through Bluetooth, and the method comprises the following steps: after the vehicle control terminal detects that the vehicle is flamed out, the vehicle control terminal detects a Bluetooth connection state with a current user terminal; when the vehicle control terminal detects that the Bluetooth connection state of the vehicle control terminal and a current user terminal is abnormal, acquiring flameout duration of the vehicle; and when the flameout duration reaches a preset threshold, the vehicle control terminal controls the vehicle to enter a fortification mode. According to the invention, the vehicle can be fortified even if the Bluetooth connection between the current user terminal and the vehicle control terminal is interrupted or the connection signal is poor, so that the vehicle safety is improved.

摘要：【中文】本发明公开了一种胸腔闭式引流瓶固定架体，属于医用设备领域，用于胸腔引流，其包括筒体，所述筒体内设置有束缚带，束缚带为扁平状闭环带体，束缚带的一端穿过筒体上的束缚带调节孔后固定于转轴上，转轴的两端通过通过轴承结构安装于转轴座体上，转轴座体位于筒体的外侧；所述转轴在与转轴座体的连接位置处设置有扭簧；所述的筒体的底部设置有矩阵分布的插接杆。鉴于上述技术方案，本发明能够适应于不同应用场景下的引流瓶瓶体固定，且能够提高引流瓶放置架体的灵活性及便捷性。 【EN】The invention discloses a fixing frame body of a thoracic cavity closed drainage bottle, which belongs to the field of medical equipment and is used for thoracic cavity drainage, and the fixing frame body comprises a barrel body, wherein a binding belt is arranged in the barrel body, the binding belt is a flat closed-loop belt body, one end of the binding belt penetrates through a binding belt adjusting hole on the barrel body and then is fixed on a rotating shaft, two ends of the rotating shaft are arranged on a rotating shaft seat body through a bearing structure, and the rotating shaft seat body is positioned on the outer side of the barrel body; a torsion spring is arranged at the connecting position of the rotating shaft and the rotating shaft seat body; the bottom of the cylinder body is provided with insertion rods distributed in a matrix manner. In view of the technical scheme, the drainage bottle fixing device can be suitable for fixing the drainage bottle bodies in different application scenes, and the flexibility and the convenience of the drainage bottle placing frame body can be improved.

摘要：【中文】一种复合波杂发酵饮料的制备方法，属于食品加工技术领域。本发明将玉米粉、大米粉、小米粉原料混合与水煮制后组成的谷物原浆作为发酵基质进行发酵，选用复合菌剂进行复配发酵，得到复合波杂发酵饮料成品。本发明通过复合发酵法制备波杂发酵饮料，所述制备方法工艺简单，可大大缩短发酵时间及提高谷物利用率，制得的波杂发酵饮料酸甜可口，口感细腻，具有谷物与果香复合的香气，适用于大规模工业化推广、应用和生产。 【EN】A preparation method of a composite mixed fermented beverage belongs to the technical field of food processing. The invention takes corn protoplasm formed by mixing corn flour, rice flour and millet flour raw materials and boiling the raw materials with water as a fermentation substrate for fermentation, and selects a compound microbial inoculum for compound fermentation to obtain a finished product of the compound wave and mixed fermented beverage. The invention prepares the fermented drink with the mixture by the composite fermentation method, the preparation method has simple process, can greatly shorten the fermentation time and improve the utilization rate of grains, and the prepared fermented drink with the mixture is sour, sweet and delicious, has fine and smooth mouthfeel, has the fragrance of the grains and the fruity fragrance, and is suitable for large-scale industrial popularization, application and production.

摘要：【中文】本发明公开了一种手性β‑羟基酰胺类化合物及其制备方法。该化合物如式I所示。本发明提供的制备非天然手性氨基酸类合物的原料是用红球菌Rhodococcus erythropolis AJ270微生物体系催化水解带有不同取代基的前手性二酰胺类化合物II得到。所用红球菌菌体用量可根据底物的用量来进行调节。反应溶剂为pH值6.0‑8.0的常用缓冲溶液，温度为20‑37℃，反应时间为3‑120小时。该红球菌微生物催化体系具有可发酵培养和保存方便的特点。运用此生物转化制备手性单酰胺羧酸、二羧酸的方法，具有操作简便，反应高效，反应条件温和，对映选择性高，产物易分离，产物纯度高的特点，具有很好的应用前景。 【EN】The invention discloses a chiral β -hydroxy amide compound and a preparation method thereof, wherein the compound is shown as a formula I, and the raw material for preparing the non-natural chiral amino acid compound provided by the invention is Rhodococcus erythropolis AJ270The microbial system catalyzes and hydrolyzes prochiral diamide compounds II with different substituents to obtain the compound. The dosage of the rhodococcus thallus can be adjusted according to the dosage of the substrate. The reaction solvent is common buffer solution with pH value of 6.0-8.0, the temperature is 20-37 ℃, and the reaction time is 3-120 hours. The Rhodococcus microorganism catalysis system has the characteristics of fermentation culture and convenient preservation. The method for preparing the chiral monoamide carboxylic acid and the chiral dicarboxylic acid by biotransformation has the characteristics of simple and convenient operation, high reaction efficiency, mild reaction conditions, high enantioselectivity, easy separation of products and high product purity, and has good application prospect.