摘要：【中文】本发明公开了一种基于微生物组测序数据和机器学习算法的死亡时间推断方法，以提取自小鼠尸体的盲肠样本中的DNA为模板，针对微生物的16S rDNA设计引物进行PCR扩增，利用扩增产物建立基因文库并测序。对有效测序数据进行OTUs聚类和物种分类分析。基于物种的丰度分布情况去除相对丰度及组间方差变化小的物种分类，提取得到用来建立死亡时间推断模型的特征微生物种类，利用小鼠死亡后不同时间点的样本微生物丰度数据训练人工神经网络，得到可以准确预测死亡时间的数学模型。 【EN】The invention discloses a dead time inference method based on microbiome sequencing data and a machine learning algorithm, which takes DNA extracted from a cecum sample of a mouse carcass as a template, designs a primer aiming at 16S rDNA of a microorganism for PCR amplification, establishes a gene library by using an amplification product and sequences. And carrying out OTUS clustering and species classification analysis on the effective sequencing data. Removing species classification with small relative abundance and small variance change among groups based on the abundance distribution condition of the species, extracting to obtain a characteristic microorganism species for establishing a death time inference model, training an artificial neural network by utilizing sample microorganism abundance data of different time points after the death of the mouse, and obtaining a mathematical model capable of accurately predicting the death time.

摘要：【中文】本发明提供的一种圆柱形电芯单边焊接的方法，包括多个电芯、汇流排、上支架、下支架、铝丝、电芯正极、电芯负极和电芯边缘，先将所述多个电芯整齐排布于所述上、下支架之间形成电池模组，再将所述电芯正极与所述上支架固定连接，所述电芯负极与所述下支架固定连接。所述汇流排固定在所述上支架上，所述电芯正极与所述汇流排通过所述铝丝焊接连接。本发明通过上述电芯排布方式，只在正极一面装配所述汇流排，将所述汇流排分别与所述电芯正极、所述电芯边缘通过所述铝丝焊接，从而将电芯串并联连接，能够减少汇流排和铝丝等材料的使用，还能减少焊接工作量，能进一步提高电池组的散热效果。 【EN】The invention provides a unilateral welding method of a cylindrical battery cell, which comprises a plurality of battery cells, a busbar, an upper bracket, a lower bracket, an aluminum wire, a battery cell anode, a battery cell cathode and a battery cell edge, wherein the plurality of battery cells are orderly arranged between the upper bracket and the lower bracket to form a battery module, the battery cell anode is fixedly connected with the upper bracket, and the battery cell cathode is fixedly connected with the lower bracket. The busbar is fixed on the upper support, and the positive electrode of the battery cell is connected with the busbar through the aluminum wire in a welding manner. According to the battery cell arrangement mode, the bus bar is only assembled on one surface of the positive electrode, and the bus bar is respectively welded with the positive electrode of the battery cell and the edge of the battery cell through the aluminum wire, so that the battery cells are connected in series and in parallel, the use of materials such as the bus bar and the aluminum wire can be reduced, the welding workload can be reduced, and the heat dissipation effect of the battery pack can be further improved.

摘要：【中文】本发明公开了一种路桥梁施工专用灌注桩钻孔机，转动板既能水平置于固定架上方，又能翻转90°后竖直置于固定架的外侧；转动板的右侧安装有滑板、第一电机、支撑板、输送绞龙、第一电机、限位筒，限位筒的上部侧壁上开设有泥土出口，限位筒下部外壁间隙套装有孔口定位挡板组件，输送绞龙的下端伸到限位筒外，并在输送绞龙的底端固连有钻头，当移动环下降到最低位置时，所有弧板与合金刀片正好拼合为一块完整的面板，当移动环上升到最高位置时，所有弧板与合金刀片分离，从而在弧板与合金刀片之间形成供泥土通过的缝隙。该钻孔机解决了常见的钻孔机因受力不平衡导致的钻头发生倾斜，影响施工和钻头取出时底部有大量杂质，影响灌装柱性能的问题。 【EN】The invention discloses a bored concrete pile drilling machine special for road and bridge construction.A rotating plate can be horizontally arranged above a fixed frame and also can be vertically arranged outside the fixed frame after being turned over for 90 degrees; the slide is installed on the right side of rotor plate, a first motor, a supporting plate, carry the auger, a first motor, a spacing section of thick bamboo, the earth export has been seted up on the upper portion lateral wall of a spacing section of thick bamboo, spacing section of thick bamboo lower part outer wall clearance cover is equipped with drill way positioning baffle subassembly, the lower extreme of carrying the auger stretches outside spacing section of thick bamboo, and the drill bit has been linked firmly in the bottom of carrying the auger, when the shift ring descends the extreme lower position, all arc boards and alloy blade just in time splice into a complete panel, when the shift ring rose to the highest position, all arc boards and alloy blade separation, thereby form the gap that supplies earth to pass through between arc board and alloy blade. The drill solves the problems that the drill bit of a common drilling machine is inclined due to unbalanced stress, and the bottom of the common drilling machine has a large amount of impurities when the construction and the drill bit are taken out, so that the performance of a filling column is influenced.

摘要：【中文】本发明涉及一种钢筋套筒连接结构内的灌浆体密实度检测方法，其包括预压构件、传力棒、伸缩调节件、振动传感器和数据采集系统；所述传力棒为一条刚性棒体，其通过伸缩调节件安装在刚性预压构件上；所述的刚性预压构件用于将传力棒固定于钢筋套筒连接结构所在的墙体上，所述伸缩调节件固定于刚性预压构件上，用于控制传力棒沿垂直墙体方向移动，使传力棒端部紧密支顶于待检测的钢筋套筒内的钢筋表面上；所述的振动传感器固定于传力棒上，所述数据采集系统用于采集振动传感器的感应信号；所述的对钢筋套筒连接的灌浆密实度进行定量的分析，可以使用时域信号得到峰宽比，或是频域信号的最大幅值对应的频率，作为定量分析的标准。 【EN】The invention relates to a method for detecting the compactness of a grouting body in a steel bar sleeve connecting structure, which comprises a prepressing component, a force transmission rod, a telescopic adjusting part, a vibration sensor and a data acquisition system, wherein the prepressing component is arranged on the top of the steel bar sleeve; the force transmission rod is a rigid rod body and is arranged on the rigid prepressing component through a telescopic adjusting piece; the rigid prepressing component is used for fixing the force transfer rod on a wall body where the steel bar sleeve connecting structure is located, and the telescopic adjusting part is fixed on the rigid prepressing component and used for controlling the force transfer rod to move along the direction vertical to the wall body so that the end part of the force transfer rod is tightly supported on the surface of a steel bar in the steel bar sleeve to be detected; the vibration sensor is fixed on the force transmission rod, and the data acquisition system is used for acquiring sensing signals of the vibration sensor; the quantitative analysis of the grouting compactness of the steel bar sleeve connection can use a time domain signal to obtain a peak width ratio or a frequency corresponding to the maximum amplitude of a frequency domain signal as a standard of quantitative analysis.

摘要：【中文】本发明涉及一种无菌恒湿细菌培养实验箱装置，本发明适用于细菌培养过程中培养皿内部湿度的精确控制，内部实现闭环循环，不与外界接触，降低感染的风险。该实验箱由培养皿舱室、水箱和干燥管舱室、控制舱室组成，实验箱首先在超净工作台上进行杀菌，然后在超净工作台上将实验用细菌培养皿放入实验箱中，最后将整个实验箱放入恒温恒湿箱中。通过安装在培养皿舱室内部的温湿度传感器测得培养皿舱室内部的当前温湿度，控制舱室中的控制单元会根据当前湿度和用户设定的目标湿度进行比较，通过控制与水箱和干燥管舱室连接的气泵来进行加湿或除湿操作，实验过程中湿度变化状况会上传至服务器，操作者通过PC端软件和手机APP实时查询服务器上当前和历史记录的作业状态。 【EN】The invention relates to an aseptic constant humidity bacteria culture experiment box device which is suitable for accurately controlling the humidity inside a culture dish in the bacteria culture process, realizes closed loop circulation inside, does not contact with the outside and reduces the risk of infection. This experimental box comprises culture dish cabin, water tank and drying tube cabin, control cabin, and the experimental box disinfects on the superclean bench at first, then puts into the experimental box with the experiment with the bacterial culture dish on the superclean bench in, puts into constant temperature and humidity case with whole experimental box at last. The current humiture in the culture dish cabin is measured through installing the humiture sensor in the culture dish cabin inside, the control unit in the control cabin can carry out the comparison according to current humidity and the target humidity that the user set for, carry out humidification or dehumidification operation through the air pump of control and water tank and drying tube cabin connection, humidity change situation can upload to the server in the experimentation, the operator passes through PC end software and cell-phone APP real-time query server and goes up current and historical record's operation state.