摘要：【中文】本发明公开了一种强酸性条件下基质附着生物膜中微生物总DNA的提取方法，属于分子生物学技术领域。包括以下步骤：1)采用pH＝4～5的缓冲液1在适宜水压下将微生物从附着基质上洗脱，离心收集菌体样品；2)采用含有EDTA的pH＝7～7.5的缓冲液2进行洗涤、离心收集菌体样品；3)将步骤2)收集的菌体样品进行DNA提取。本发明的方法提取的微生物总核糖核酸质量较好，采用1％琼脂糖凝胶电泳检测得到的总条带明亮清晰，上机测序时DNA链长和浓度符合测序和建库要求，为强酸性条件下微生物生态学和分子生物学研究奠定了技术基础。 【EN】The invention discloses a method for extracting total DNA of microorganisms in a substrate-attached biomembrane under a strong acid condition, and belongs to the technical field of molecular biology. The method comprises the following steps: 1) eluting microorganisms from the attachment matrix by using a buffer solution 1 with the pH value of 4-5 under proper water pressure, and centrifugally collecting a thallus sample; 2) washing with a buffer solution 2 containing EDTA and having a pH value of 7-7.5, and centrifuging to collect a thallus sample; 3) extracting DNA from the thallus sample collected in the step 2). The microbial total ribonucleic acid extracted by the method has good quality, the total band obtained by detecting the method by adopting 1 percent agarose gel electrophoresis is bright and clear, the length and the concentration of the DNA chain meet the requirements of sequencing and library building when the DNA is subjected to on-machine sequencing, and the method lays a technical foundation for microbial ecology and molecular biology research under the strong acid condition.

摘要：【中文】本发明公开了一种提取强酸性条件下基质微生物总RNA和总蛋白的方法，属于分子生物学技术领域。本发明的方法包括以下步骤：1)取基质材料，采用pH＝3.6～5.5的缓冲溶液A在一定压力的水压下冲洗基质材料，将微生物从附着基质表面上洗脱，离心收集菌体；2)将步骤1)收集的菌体裂解，采用溶液B提取总RNA和总蛋白，所述溶液B为pH＝4.0～4.5的Trizol试剂。所述水压压力范围为30～120MPa。本发明提取方法显著改善了强酸性条件下基质附着微生物总RNA和总蛋白的提取效率和质量，满足同时提取或分别提取RNA和蛋白质的检测要求。 【EN】The invention discloses a method for extracting total RNA and total protein of matrix microorganisms under a strong acid condition, and belongs to the technical field of molecular biology. The method of the invention comprises the following steps: 1) taking a matrix material, adopting a buffer solution A with the pH value of 3.6-5.5 to wash the matrix material under the water pressure of certain pressure, eluting microorganisms from the surface of an attached matrix, and centrifugally collecting thalli; 2) cracking the thallus collected in the step 1), and extracting total RNA and total protein by using a solution B, wherein the solution B is a Trizol reagent with the pH value of 4.0-4.5. The water pressure range is 30-120 MPa. The extraction method of the invention obviously improves the extraction efficiency and quality of the total RNA and the total protein of the matrix-attached microorganisms under the strong acid condition, and meets the detection requirements of simultaneously extracting or respectively extracting the RNA and the protein.

摘要：【中文】本发明提供一种荧光灯管含汞固废无害化处理方法，属于固废处理技术领域，本发明是将灯管碎片装入密封罐中利用超声波对含汞灯管碎片进行预处理，再采用清洗液、含酸溶液和纯水依次进行洗涤，最后在密封罐外部连接等离子发生设备，利用等离子气体对清洗后的灯管表面进行后处理，不仅可以进一步清除灯管碎片表面的残余汞而且还可以对灯管碎片进行烘干，将废旧荧光灯管汞的浸出液含量控制在0.02mg/L以下。 【EN】The invention provides a harmless treatment method for mercury-containing solid waste of a fluorescent lamp tube, belonging to the technical field of solid waste treatment, the method comprises the steps of putting lamp tube fragments into a sealed tank, pretreating the mercury-containing lamp tube fragments by using ultrasonic waves, washing the lamp tube fragments by using a cleaning solution, an acid-containing solution and pure water in sequence, connecting plasma generation equipment outside the sealed tank, and carrying out post-treatment on the surface of the cleaned lamp tube by using plasma gas, so that residual mercury on the surface of the lamp tube fragments can be further removed, the lamp tube fragments can be dried, and the content of a leaching solution of mercury in the waste fluorescent lamp tube is controlled to be below 0.02 mg/L.

摘要：【中文】本发明提供一种基于虚拟化技术的卫星接收站监控系统，包括虚拟化硬件平台和虚拟化传输网络；虚拟化硬件平台包括第一磁盘阵列、第二磁盘阵列、第一光纤交换机、第二光纤交换机、多台服务器组成的服务器虚拟资源池；虚拟化传输网络包括运行在每一台所述服务器上的虚拟机和虚拟交换机，虚拟机具有虚拟网卡；每一服务器中，虚拟交换机的下层数据接口与虚拟网卡连接，虚拟交换机的上层数据接口与物理网卡连接。以上方案，采用虚拟化技术，将服务器、存储、网络等物理资源整合成统一的逻辑资源池，通过虚拟化网络设计、虚拟化存储双活设计，解决了服务器、存储、网络的单点瓶颈问题，提高了系统资源的利用率、高可用性和可扩展性。 【EN】The invention provides a satellite receiving station monitoring system based on virtualization technology, which comprises a virtualization hardware platform and a virtualization transmission network, wherein the virtualization hardware platform is used for storing a plurality of virtual hardware; the virtualization hardware platform comprises a server virtual resource pool consisting of a first disk array, a second disk array, a first optical fiber switch, a second optical fiber switch and a plurality of servers; the virtual transmission network comprises a virtual machine and a virtual switch which run on each server, and the virtual machine is provided with a virtual network card; in each server, a lower data interface of the virtual switch is connected with the virtual network card, and an upper data interface of the virtual switch is connected with the physical network card. The scheme adopts the virtualization technology to integrate physical resources such as servers, storage, networks and the like into a unified logical resource pool, solves the single-point bottleneck problem of the servers, the storage and the networks through the virtualization network design and the virtualization storage dual-active design, and improves the utilization rate, the high availability and the expandability of system resources.

摘要：【中文】本发明公开了一种提取基质附着微生物总DNA的装置和方法，属于分子生物学技术领域，该装置包括控压冲洗装置、负压装置和收集装置，通过控压冲洗装置控制冲洗出水水压，通过负压管路将冲洗管路冲洗基质产生的混悬液收集至收集装置，本发明同时公开了DNA的提取方法，在加压下冲洗基质，配合负压装置收集样品，克服了现有技术针对附着在基质上的微生物样品存在取样困难、取样不完整的难题，显著改善了基质附着微生物基因组DNA的代表性、提取效率和质量，条带明亮清晰、基本无降解，对难提取微生物，如真菌等均有较好的提取效率。 【EN】The invention discloses a device and a method for extracting total DNA of matrix-attached microorganisms, belonging to the technical field of molecular biology.

摘要：【中文】本发明公开了贝雷架保护电缆的方法和系统，核心步骤在于：在电缆管沟的两侧开挖沟槽，在所述贝雷架基础上设置贝雷架，在所述沟槽的内放置所述贝雷架的悬吊结构；选取所述地铁基坑的其中一个侧面开始，沿所述电缆管沟的长度方向分节段逐级开挖所述电缆管沟的下方土体，在每一节段的所述电缆管沟的下方土体开挖完成后，在所述节段对应的所述电缆管沟的部分下方架设横向型钢，将所述横向型钢与所述贝雷架的悬吊结构焊接固定后再开挖下一所述节段的所述电缆管沟的下方土体，直至开挖到所述地铁基坑的另一个侧面；在地铁施工与电缆设置发生冲突时，如该电缆无法进行迁改，通过本发明提供的方法以及系统能够有效使电缆在地铁基坑挖掘的过程中得到保护。 【EN】The invention discloses a method and a system for protecting a cable by a bailey truss, which have the core steps that: grooves are dug on two sides of a cable pipe trench, a bailey frame is arranged on the basis of the bailey frame, and a suspension structure of the bailey frame is placed in the grooves; selecting one side surface of the subway foundation pit, excavating the soil body below the cable pipe trench in sections step by step along the length direction of the cable pipe trench, erecting transverse section steel below the part, corresponding to each section, of the cable pipe trench after the excavation of the soil body below the cable pipe trench of each section is finished, and excavating the soil body below the cable pipe trench of the next section after the transverse section steel is welded and fixed with the suspension structure of the bailey frame until the other side surface of the subway foundation pit is excavated; when the subway construction conflicts with the cable arrangement, if the cable cannot be moved and changed, the method and the system provided by the invention can effectively protect the cable in the excavation process of the subway foundation pit.

摘要：【中文】本发明公开了跨地铁迁改电缆的方法和系统，步骤包括：在地铁隧道位置的上方且在不超过路面的区域内设置桥架，所述桥架的两端通过连接基座与地铁隧道位置上方的区域实现桥架固定，在桥架内部沿桥架的长度方向放置若干个电缆通道，并将电缆通道两端的开口分别置于桥架的两端并超出桥架的两端，使电缆通道连通整个桥架，在电缆通道中敷设跨桥电缆；在位于地铁位置以外的桥架的两端处分别开设电缆连接井，在电缆连接井内设置有至少两个连接井通道，其中一个连接井通道与电缆通道相连通，另一个连接井通道导入迁改电缆的一端，使迁改电缆与跨桥电缆在电缆连接井中接通，使迁改电缆两端通过跨桥电缆跨过地铁隧道连通，该方法费用更低，耗时少且风险低。 【EN】The invention discloses a method and a system for changing a cable across subways, which comprises the following steps: arranging a bridge frame above the subway tunnel and in an area not exceeding a road surface, fixing the bridge frame at two ends of the bridge frame and the area above the subway tunnel through a connecting base, placing a plurality of cable channels in the bridge frame along the length direction of the bridge frame, respectively placing openings at two ends of the cable channels at two ends of the bridge frame and exceeding the two ends of the bridge frame, enabling the cable channels to be communicated with the whole bridge frame, and laying a bridge-crossing cable in the cable channels; the method is characterized in that cable connecting wells are respectively arranged at two ends of the bridge frame outside the subway position, at least two connecting well channels are arranged in the cable connecting wells, one connecting well channel is communicated with the cable channel, one end of a moving cable is led into the other connecting well channel, the moving cable is communicated with a bridge-crossing cable in the cable connecting well, and two ends of the moving cable are communicated across the subway tunnel through the bridge-crossing cable.

摘要：【中文】本发明公开了一个保护电缆的方法和系统，步骤包括：S1：在地铁基坑开挖前对电缆管沟测量定位，开挖电缆管沟上方的土体；S2：在电缆管沟的两端分别施做基坑连续墙并对基坑连续墙的外侧作加固处理；S3：在电缆管沟的两侧施做混凝土连梁立柱；S4：沿混凝土连梁立柱的排列方向开挖沟槽，设置混凝土钢筋；S5：分节段开挖电缆管沟的下方土体，在每一节段的电缆管沟的下方架设横向型钢，将横向型钢与混凝土钢筋焊接固定后继续开挖，直至地铁基坑的另一侧面；S6：浇筑混凝土钢筋形成混凝土连梁并施做冠梁；S7：在冠梁的下方逐级开挖形成地铁基坑，在每一节段开挖完成后，在每一个基坑连续墙上分别作加固处理后再开挖下一节段，直至开挖到地铁基坑的底面。 【EN】The invention discloses a method and a system for protecting cables, which comprises the following steps: s1: measuring and positioning the cable pipe trench before excavation of the subway foundation pit, and excavating a soil body above the cable pipe trench; s2: constructing a foundation pit continuous wall at two ends of the cable pipe trench respectively and reinforcing the outer side of the foundation pit continuous wall; s3: constructing concrete connecting beam upright columns on two sides of the cable trench; s4: digging grooves along the arrangement direction of the concrete connecting beam columns, and arranging concrete reinforcing steel bars; s5: excavating a soil body below the cable pipe trench in sections, erecting transverse section steel below the cable pipe trench of each section, and continuously excavating after the transverse section steel and concrete reinforcing steel bars are welded and fixed until the other side surface of the subway foundation pit; s6: pouring concrete steel bars to form concrete coupling beams and constructing crown beams; s7: and excavating step by step below the crown beam to form a subway foundation pit, and after each section of the foundation pit is excavated, respectively reinforcing each continuous wall of the foundation pit and then excavating the next section of the foundation pit until the next section of the foundation pit is excavated to the bottom surface of the subway foundation pit.

摘要：【中文】本发明涉及一种防道路基层开裂的底基层结构，其特征在于：所述底基层是使用废弃橡胶粉改性水泥稳定碎石代替传统的级配碎石或半刚性的水泥稳定碎石作为底基层，底基层按重量计矿料配合比为：10‑30mm碎石、10‑20mm碎石、5‑10mm碎石、石硝、废弃橡胶粉的组成比例为15%‑25%、20%‑27%、15%‑25%、17%‑21%、2%‑12%；按矿料总重量的3%‑7%的剂量添加水泥，水泥类型和强度等级是42.5的普通硅酸盐水泥；废弃橡胶粉粒径为40‑60目，底基层厚度为15‑25cm，底基层设计强度3.0‑5.0MPa。本发明使用废弃橡胶粉改性水泥稳定碎石作为底基层，减少了对上部下基层的约束作用，降低了上部下基层底部拉应力和拉应变，有效防止了道路基层开裂。 【EN】The invention relates to a sub-base structure for preventing a road base layer from cracking, which is characterized in that: the subbase uses the waste rubber powder modified cement stabilized macadam to replace the traditional graded macadam or semi-rigid cement stabilized macadam as the subbase, and the subbase comprises the following mineral aggregate in proportion by weight: the composition proportion of 10-30mm broken stone, 10-20mm broken stone, 5-10mm broken stone, stone nitrate and waste rubber powder is 15% -25%, 20% -27%, 15% -25%, 17% -21% and 2% -12%; adding cement in an amount of 3-7% by weight of the total weight of the mineral aggregate, ordinary portland cement of a cement type and strength grade of 42.5; the particle size of the waste rubber powder is 40-60 meshes, the thickness of the subbase layer is 15-25cm, and the design strength of the subbase layer is 3.0-5.0 MPa. The invention uses the waste rubber powder modified cement stabilized macadam as the subbase, reduces the constraint effect on the upper lower base layer, reduces the tensile stress and the tensile strain of the bottom of the upper lower base layer, and effectively prevents the road base layer from cracking.

摘要：【中文】本发明涉及一种新型道路基层结构，包括上基层（3）和下基层（4），其特征在于：基层强度为6.0‑9.0MPa，上基层（3）为废弃橡胶改性水泥稳定碎石，下基层（4）为水泥稳定碎石，上基层（3）按重量百分比，矿料配合比为：10‑30mm碎石、10‑20mm碎石、5‑10mm碎石、石硝、废弃橡胶粉的组成比例为15%‑25%、20%‑27%、15%‑25%、17%‑21%、2%‑12%；按矿料总重量的5%‑10%的剂量添加水泥，上基层（3）厚度为15‑25cm，下基层（4）按重量百分比，矿料配合比为：10‑30mm碎石、10‑20mm碎石、5‑10mm碎石、石硝的组成比例为15%‑25%、20%‑27%、15%‑25%、23%‑33%；按矿料总重量的5‑7%的剂量添加水泥，下基层（4）厚度为15‑25cm。本发明可减少沥青面层底部拉应力和拉应变，降低对车轮的敏感性，减少下基层开裂引发反射裂缝，降低沥青面层出现裂缝。 【EN】The invention relates to a novel road base layer structure, which comprises an upper base layer (3) and a lower base layer (4), and is characterized in that: the base course has the strength of 6.0-9.0MPa, the upper base course (3) is waste rubber modified cement stabilized macadam, the lower base course (4) is cement stabilized macadam, the upper base course (3) comprises the following mineral aggregate in percentage by weight: the composition proportion of 10-30mm broken stone, 10-20mm broken stone, 5-10mm broken stone, stone nitrate and waste rubber powder is 15% -25%, 20% -27%, 15% -25%, 17% -21% and 2% -12%; adding cement in an amount which is 5-10% of the total weight of the mineral aggregate, wherein the thickness of the upper base layer (3) is 15-25cm, the lower base layer (4) comprises the following mineral aggregate in percentage by weight: the composition proportion of 10-30mm broken stone, 10-20mm broken stone, 5-10mm broken stone and stone nitrate is 15% -25%, 20% -27%, 15% -25% and 23% -33%; the cement is added according to the dosage of 5-7% of the total weight of the mineral aggregate, and the thickness of the lower base layer (4) is 15-25 cm. The invention can reduce the tensile stress and the tensile strain at the bottom of the asphalt surface layer, reduce the sensitivity to wheels, reduce the reflection cracks caused by the cracking of the lower base layer and reduce the cracks of the asphalt surface layer.