摘要：【中文】本发明公开了一种基于苯并噻唑联杂环的半花菁类化合物及其制备方法与应用。该类化合物的结构式式如式I所示：该化合物的制备方法，包括如下步骤：以式Ⅱ所示的苯并噻唑类化合物与卤代烷R^bX进行烷基化反应，得到取代的苯并噻唑类化合物，然后与N‑乙基咔唑‑3‑甲醛进行Knoevenagel缩合反应，即得。本发明合成制备步骤简单易行、原料廉价易得，作为探针结构稳定、具有高特异性，可用于特异性检测核酸的G‑四链体和双螺旋的二级结构。所述探针对核酸二级结构的检测方法具有价格低廉、操作简便，选择性好，快捷的优点，克服了其他检测方法价格昂贵、设备要求高及技术操作相对复杂的缺点。 【EN】The invention discloses a hemicyanine compound based on benzothiazole linked heterocycle and a preparation method and application thereof. The structural formula of the compound is shown as formula I:the preparation method of the compound comprises the following steps: benzothiazole compound shown as formula II and alkyl halide R^bPerforming alkylation reaction on the X to obtain a substituted benzothiazole compound, and then performing Kno reaction on the substituted benzothiazole compound and N-ethylcarbazole-3-formaldehydeAnd performing evenagel condensation reaction to obtain the compound. The invention has simple and easy synthetic preparation steps, cheap and easily obtained raw materials, stable structure as a probe, high specificity and capability of being used for specifically detecting the secondary structure of G-quadruplex and double helix of nucleic acid. The method for detecting the secondary structure of the nucleic acid by the probe has the advantages of low price, simple and convenient operation, good selectivity and rapidness, and overcomes the defects of high price, high equipment requirement and relatively complex technical operation of other detection methods.

摘要：【中文】本发明涉及食品加工领域，具体涉及一种黑茶风味速溶粉的制备方法，包括以下步骤：S1.将黑茶与含有抗氧化活性物质的植物原料混合粉碎得到初料，加入提取剂提取；S2.在步骤S1得到的滤渣中加入复合菌发酵，所述复合菌为保加利亚乳杆菌和嗜热链球菌，其中保加利亚乳杆菌和嗜热链球菌的菌落数比为1：(3‑5)；S3.将步骤S2得到的发酵物灭菌，再将加入步骤S1所得到的提取物得到分散液；S4.将阿拉伯树胶、卡拉胶、麦芽糊精混合再加入步骤S3的分散液搅拌均匀后喷雾干燥。本发明的制备方法获得的速溶粉具有更好的清除自由基以及提高免疫力的效果，此外，还具有更长的保存期限。 【EN】The invention relates to the field of food processing, in particular to a preparation method of instant powder with a black tea flavor, which comprises the following steps: s1, mixing and crushing black tea and plant raw materials containing antioxidant active substances to obtain primary materials, and adding an extracting agent for extraction; s2, adding composite bacteria into the filter residue obtained in the step S1 for fermentation, wherein the composite bacteria are Lactobacillus bulgaricus and Streptococcus thermophilus, and the colony number ratio of the Lactobacillus bulgaricus to the Streptococcus thermophilus is 1: (3-5); s3, sterilizing the fermentation product obtained in the step S2, and adding the extract obtained in the step S1 to obtain a dispersion liquid; s4, mixing the gum arabic, the carrageenan and the maltodextrin, adding the dispersion liquid obtained in the step S3, uniformly stirring, and then carrying out spray drying. The instant powder obtained by the preparation method has better effects of scavenging free radicals and improving immunity, and has longer shelf life.

摘要：【中文】本发明涉及一种电网线路防非同期合环的方法，包括如下步骤：首先，判断需停检变压器的接线组别；然后，判断和需停检变压器可构成环路的备用变压器的接线组别，若两者接线组别相同，则判定为可合环操作，并在SCADA调度系统上的主接线图上预闭合备用变压器下行的热备用开关，进行拓扑结构校验；校验通过后，示意可进行合环实操。本发明能够有效解决现有的个别线路同期系统在并网时，经常出现较大角度合闸情况的问题。 【EN】The invention relates to a method for preventing asynchronous loop closing of a power grid line, which comprises the following steps: firstly, judging the wiring group of the transformer needing to be stopped and checked; then, judging the wiring group of a standby transformer which can form a loop with the transformer needing to be stopped and checked, if the wiring group of the standby transformer is the same as that of the standby transformer, judging that loop closing operation can be performed, pre-closing a hot standby switch at the downlink of the standby transformer on a main wiring diagram on an SCADA (supervisory control and data acquisition) scheduling system, and checking a topological structure; after the check is passed, the loop closing operation can be performed. The invention can effectively solve the problem that the existing individual line synchronization system is frequently switched on at a larger angle when being connected to the grid.