摘要：【中文】本发明公开了一种大型引射换热式低温环境风洞，包括风洞和所述风洞内设置的截面矩阵式喷头及风机，所述风洞为封闭环状通道，所述截面矩阵式喷头和所述风机均设置在所述风洞的封闭环状通道内，所述风洞内设有试验段，所述试验段用于进行试验件的风洞试验，所述截面矩阵式喷头连通有进气管道，所述进气管道用于通入制冷气体，所述风机位于所述截面矩阵式喷头的上游，所述风机能够驱动所述风洞内的空气循环流动，所述风洞上开设有排气口，所述排气口处设置有排气阀。本发明能够实现低温高速风洞环境的模拟，同时能够显著减小风洞内阻力，提高能量利用率，减少风洞体积和占用空间，达到节能减排和降低建造维护成本的目的。 【EN】The invention discloses a large-scale injection heat exchange type low-temperature environment wind tunnel which comprises a wind tunnel, a section matrix type spray head and a fan, wherein the section matrix type spray head and the fan are arranged in the wind tunnel, the wind tunnel is a closed annular channel, the section matrix type spray head and the fan are both arranged in the closed annular channel of the wind tunnel, a test section is arranged in the wind tunnel and used for performing a wind tunnel test on a test piece, the section matrix type spray head is communicated with an air inlet pipeline, the air inlet pipeline is used for introducing refrigeration gas, the fan is positioned at the upstream of the section matrix type spray head and can drive air in the wind tunnel to circularly flow, an exhaust port is formed in the wind tunnel, and an exhaust valve is arranged at the. The simulation system can realize the simulation of the low-temperature high-speed wind tunnel environment, can obviously reduce the internal resistance of the wind tunnel, improve the energy utilization rate, reduce the wind tunnel volume and the occupied space, and achieve the purposes of saving energy, reducing emission and reducing the construction and maintenance cost.

摘要：【中文】本发明属于生物催化技术领域，更具体地涉及一种降低氮源用量的甾体药物前体高效生产方法。该方法是通过敲除甾体药物前体生产菌株中的氮源转录调节因子glnR基因实现的。所获得的基因工程菌比改造前的原始菌株在低氮源下具有更高的生物量及菌体活力，AD的生产能力也得到加强。使用本方法描述的基因工程菌进行工业生产可在降低氮源需求的情况下提高转化率，可大大提高生产效率降低生产成本。 【EN】The invention belongs to the technical field of biological catalysis, and particularly relates to a high-efficiency production method of a steroid prodrug for reducing the dosage of a nitrogen source. The method is realized by knocking out a nitrogen source transcription regulatory factor glnR gene in a steroid prodrug production strain. Compared with the original strain before modification, the obtained genetically engineered bacterium has higher biomass and thallus activity under a low nitrogen source, and the AD production capacity is enhanced. The genetic engineering bacteria described by the method can be used for industrial production, the conversion rate can be improved under the condition of reducing the nitrogen source requirement, the production efficiency can be greatly improved, and the production cost can be greatly reduced.

摘要：【中文】本发明属于生物技术领域，公开了一种11α‑羟基‑甲基睾丸素的生物合成方法。具体涉及生产菌株为经ARTP诱变与ARTP‑LiCl复合诱变选育获得的蓝色犁头霉CGMCC 14124，生产原料为甲基睾丸素(MT)。本发明利用蓝色犁头霉CGMCC 14124的选择性羟基化能力，将甲基睾丸素加入培养成熟的发酵培养基中，可以有效合成11α‑羟基‑甲基睾丸素。本发明利用微生物转化法实现了11α‑羟基‑甲基睾丸素的绿色高效合成，简化操作，降低生产成本和环境污染。 【EN】The invention belongs to the field of biotechnology, and discloses a biosynthesis method of 11 α -hydroxy-methyltestosterone, in particular to a production strain of Absidia coerulea CGMCC14124 obtained by ARTP mutagenesis and ARTP-LiCl composite mutagenesis breeding, wherein the production raw material is Methyltestosterone (MT). The invention utilizes the selective hydroxylation capability of Absidia coerulea CGMCC14124, and adds the methyltestosterone into a fermentation medium which is cultured to be mature, so as to effectively synthesize 11 α -hydroxy-methyltestosterone.

摘要：【中文】本发明属于微生物甾体转化技术领域，公开了一种7α‑OH‑DHEA的生物合成方法。涉及生产菌株为蓝色犁头霉(Absidia coerulea)，具体为蓝色犁头霉CGMCC 14124，生产原料为脱氢表雄酮(DHEA)。利用该菌种的选择性羟基化能力对DHEA进行生物转化，可以有效合成目标产物7α‑OH‑DHEA。底物DHEA的投料浓度高达5.5g/L时，目标产物的生成率达到了45.5％。该方法实现了绿色环保的生产，为该甾体药物的工业化生产提供了基础。 【EN】The invention belongs to the technical field of microbial steroid conversion, and discloses a biosynthesis method of 7 α -OH-DHEA, which relates to a production strain of Absidia coerulea (Absidia coerulea), specifically Absidia coerulea CGMCC14124, wherein a production raw material is Dehydroepiandrosterone (DHEA). The DHEA is biologically converted by utilizing the selective hydroxylation capacity of the strain, a target product 7 α -OH-DHEA can be effectively synthesized, when the feeding concentration of a substrate DHEA is up to 5.5g/L, the generation rate of the target product reaches 45.5%, the method realizes green and environment-friendly production, and provides a foundation for the industrial production of steroid medicines.