摘要：【中文】本发明涉及一种用于检测乙酰胆碱酯酶活性的荧光探针，其特征在于该荧光探针的结构式为：其中，R1，R2，R3，R4为氢原子、氨基、甲基或乙基。本发明的荧光探针通过对聚多巴胺的吸附与解吸附发生荧光信号的变化来检测乙酰胆碱酯酶的活性。其优点在于检测限低、操作简单、成本低。 【EN】The invention relates to a fluorescent probe for detecting the activity of acetylcholinesterase, which is characterized in that the structural formula of the fluorescent probe is as follows:wherein R1, R2, R3 and R4 are hydrogen atoms, amino groups, methyl groups or ethyl groups. The fluorescent probe provided by the invention detects the activity of acetylcholinesterase by changing the fluorescence signal generated by the adsorption and desorption of polydopamine. Its advantages are low detection limit, simple operation and low cost.

摘要：【中文】本发明涉及一种检测乙酰胆碱酯酶活性用的荧光探针及其制备方法和应用。该探针材料的结构式为：其中：R为羟基或羧基。本发明通过对巯基类化合物响应产生荧光检测乙酰胆碱酯酶的活性，同时，通过荧光变化来筛选乙酰胆碱酯酶抑制剂。其优点在于对巯基具有较高的选择性和灵敏度，操作简便，成本低，并且可以重复利用。该类化合物作为荧光探针可应用于疾病诊断及农残检测等领域。 【EN】The invention relates to a fluorescent probe for detecting the activity of acetylcholinesterase, a preparation method and application thereof. The structural formula of the probe material is as follows:wherein: r is hydroxyl or carboxyl. The invention detects the activity of acetylcholinesterase by generating fluorescence in response to sulfhydryl compounds, and simultaneously screens acetylcholinesterase inhibitors by fluorescence change. Its advantages are high selectivity and sensitivity to mercapto group, simple operation, low cost and reuse. The compound can be used as a fluorescent probe in the fields of disease diagnosis, pesticide residue detection and the like.

摘要：【中文】本发明公开了一种黄精丛生芽诱导与试管苗形成的方法。以黄精种子无菌萌发后获得的初生根状茎为外植体材料，然后通过组织培养诱导获得带白色组织，经进一步诱导形成丛生芽，再通过分化培养形成无根苗，最后以无根苗培养生产大量黄精试管苗，经炼苗、移栽，获得生长健壮的黄精植株。本发明具有能够有效地提高黄精的繁殖系数，缩短其生长周期，为实现其工厂化育苗与药材规模化生产奠定了基础的有益效果。 【EN】The invention discloses a method for inducing cluster buds of polygonatum sibiricum and forming test-tube seedlings. Taking a primary rhizome obtained after the polygonatum sibiricum seeds are aseptically germinated as an explant material, then obtaining a whitish tissue through tissue culture and induction, further inducing to form cluster buds, then forming a rootless seedling through differentiation culture, finally producing a large amount of polygonatum sibiricum test-tube seedlings through rootless seedling culture, and obtaining a strong polygonatum sibiricum plant through seedling hardening and transplanting. The invention can effectively improve the propagation coefficient of the polygonatum sibiricum, shorten the growth period of the polygonatum sibiricum, and lay the foundation for realizing the industrial seedling culture and the large-scale production of medicinal materials.

摘要：【中文】本发明公开一种基于端头模板的隧道构建方法，所述方法包括步骤：根据预设间距以及钢筋直径的大小在端头模板上设置通孔；将所述端头模板固定在结构起点位置，将钢筋穿过所述端头模板上的通孔通过套筒与前端的钢筋连接；对所述钢筋进行绑扎后浇筑混凝土，形成隧道内钢筋混凝土结构。本发明通过在端头模板预设通孔，使得钢筋通过所述通孔排布定位以及端头钢筋预留的搭接长度精准，钢筋间距控制简单，提高施工质量，同时所述端头模板还具有封模作用，可加快施工效率，所述端头模板可循环使用，节省成本。 【EN】The invention discloses a tunnel construction method based on an end template, which comprises the following steps: arranging through holes on the end template according to the preset space and the diameter of the steel bar; fixing the end head template at the starting point of the structure, and connecting a reinforcing steel bar with the reinforcing steel bar at the front end through a sleeve after the reinforcing steel bar passes through the through hole in the end head template; and pouring concrete after binding the reinforcing steel bars to form a reinforced concrete structure in the tunnel. According to the invention, through the through holes are preset in the end template, the arrangement and positioning of the steel bars through the through holes and the overlap length reserved for the end steel bars are accurate, the control of the space between the steel bars is simple, the construction quality is improved, meanwhile, the end template also has a mold sealing effect, the construction efficiency can be accelerated, the end template can be recycled, and the cost is saved.

摘要：【中文】本发明公开了一种高效促进狐臭柴种子萌发的方法。包括有以下步骤：取成熟的狐臭柴果实，清水浸泡后，去掉果皮，清洗后，选出干净种子，备用；将备用种子浸泡在硫酸溶液中3‑8min，用清水冲洗干净，然后用滤纸吸干种子表面水分，备用；将备用种子置于赤霉素溶液中浸泡后取出，清水冲洗干净后，用滤纸吸干种子表面水分，备用；将备用种子用75％酒精、0.1％升汞和无菌水冲洗数进行消毒处理，得到消毒处理后的种子，备用；将消毒处理后的种子接种于MS培养基中进行萌发培养。本发明具有能够有效地解决狐臭柴种子因物理休眠引起的萌发困难，提高狐臭柴种子的萌发率，缩短了萌发时间，从而为其规模化生产技术提供保障，且操作简单、成本低的有益效果。 【EN】The invention discloses a method for efficiently promoting germination of bromhidrosis firewood seeds. The method comprises the following steps: soaking mature wood fruit with bromhidrosis in clear water, removing peel, cleaning, and selecting clean seeds; soaking the seeds for later use in sulfuric acid solution for 3-8min, washing with clear water, and then sucking dry water on the surface of the seeds with filter paper for later use; soaking the seeds for later use in gibberellin solution, taking out, washing with clear water, and absorbing water on the surfaces of the seeds with filter paper for later use; sterilizing the seeds for later use by using 75% of alcohol, 0.1% of mercury bichloride and sterile water for washing to obtain sterilized seeds for later use; inoculating the disinfected seeds into an MS culture medium for germination culture. The method has the advantages of effectively solving the problem of difficult germination of the corymbose odoriphaga seeds caused by physical dormancy, improving the germination rate of the corymbose odoriphaga seeds and shortening the germination time, thereby providing guarantee for a large-scale production technology, along with simple operation and low cost.

摘要：【中文】本发明公开了一种用于保护地铁桥墩的钢管桩施工方法，所述方法包括根据待挖隧道边缘线确定保护墙位置，并在所述保护墙位置施工保护墙，以将地铁桥墩与待挖隧道隔离；在地铁桥墩周围施工袖阀管，以使得地铁桥墩周围均布置有袖阀管。本发明通过保护墙将高架桥墩基础范围的土体进行隔断，避免隧道开挖过程中土压不平衡而造成高架桥墩基础土方流失，防止桥墩基础侧压力不平衡而造成侧向变形，再通过袖阀管注浆包裹地铁桥墩，可避免桥墩基础范围内的水流失，对高架桥墩基础起到预加固的作用。 【EN】The invention discloses a steel pipe pile construction method for protecting subway piers, which comprises the steps of determining the position of a protection wall according to an edge line of a tunnel to be excavated, and constructing the protection wall at the position of the protection wall so as to isolate the subway piers from the tunnel to be excavated; and constructing sleeve valve pipes around the subway piers so as to ensure that the sleeve valve pipes are uniformly distributed around the subway piers. According to the invention, the soil body in the range of the viaduct pier foundation is cut off through the protection wall, so that the earthwork loss of the viaduct pier foundation caused by unbalanced soil pressure in the tunnel excavation process is avoided, the lateral deformation caused by unbalanced lateral pressure of the pier foundation is prevented, and the subway pier is wrapped by grouting through the sleeve valve pipe, so that the water loss in the range of the pier foundation can be avoided, and the function of pre-reinforcing the viaduct pier foundation is achieved.

摘要：【中文】本发明公开了一种电气试验用电力测试夹，包括插头和夹持臂，还包括夹片和夹持件。插头的数量为两个且均安装夹持臂，夹持臂相向的一侧均设置相对应的凸起，两个夹持臂通过凸起之间安装第一紧固件转动连接，两个夹持臂相向的一侧均开设安装槽，安装槽位于夹持臂的最远端的位置通过第二紧固件安装夹片，两个夹片相向的一侧均设置两个夹持件，夹持件通过第一紧固件安装在夹持臂上；电气试验用电力测试夹共有四个夹持件，四个夹持件与线夹的压力之间满足角度的可调节性。通过将插头设置外螺纹，插头远离夹持臂的一端通过螺纹配合的方式安装筒夹，解决了尾端为插拔式设计出现尾端与测试线之间夹力变小、接触不良造成重复性试验且高空挂线时容易发生坠落的问题。 【EN】The invention discloses an electric test clamp for an electrical test, which comprises a plug, a clamping arm, a clamping piece and a clamping piece. The number of the plugs is two, the clamping arms are arranged on the plugs, corresponding bulges are arranged on opposite sides of the clamping arms, the two clamping arms are rotatably connected through a first fastening piece arranged between the bulges, mounting grooves are formed in opposite sides of the two clamping arms, clamping pieces are arranged at the positions, located at the farthest ends of the clamping arms, of the mounting grooves through second fastening pieces, two clamping pieces are arranged on opposite sides of the two clamping pieces, and the clamping pieces are arranged on the clamping arms through the first fastening pieces; the electric test is with four holders altogether for the electric test clamp, satisfies the controllability of angle between the pressure of four holders and fastener. Through setting up the external screw thread with the plug, the collet chuck is installed through screw-thread fit's mode to the one end that the centre gripping arm was kept away from to the plug, has solved the tail end and has appeared for plug-in design that the clamping force between tail end and the test wire diminishes, contact failure causes the problem that takes place to fall easily when repeatability test and high altitude hanging wire.