摘要：【中文】本发明公开了一种黄瓜果实刺瘤石蜡切片制作方法，其包括如下步骤：(1)选取合适发育时期的黄瓜果实刺瘤材料立即放入装有FAA固定液的容器瓶中，轻晃动容器瓶至少一次，待黄瓜果实刺瘤材料沉于液面后固定30min；(2)然后将容器瓶中的FAA固定液全部倒掉，再次加入新的FAA固定液到容器瓶中，然后用封口膜封住瓶口，固定至少48h；(3)然后将容器瓶中的FAA固定液再次全部倒掉，加入浓度50％乙醇到容器瓶中诱导1h；(4)最后依次进行整染、脱水、透明、浸蜡与包埋、切片及粘片及展片、脱蜡与染色、封片。通过本发明获得的切片组织完整，结构清晰，可操作性和实用性强，是观察果实刺瘤解剖学结构的有效方法。 【EN】The invention discloses a method for making cucumber fruit thorn tumor paraffin slice, which comprises the following steps: (1) selecting cucumber fruit burr materials in a proper development period, immediately putting the cucumber fruit burr materials into a container bottle filled with FAA fixing liquid, slightly shaking the container bottle for at least one time, and fixing for 30min after the cucumber fruit burr materials are settled on the liquid level; (2) pouring all FAA stationary liquid in the container bottle, adding new FAA stationary liquid into the container bottle again, sealing the bottle mouth with a sealing film, and fixing for at least 48 h; (3) then pouring all FAA stationary liquid in the container bottle again, adding ethanol with the concentration of 50% into the container bottle, and inducing for 1 h; (4) and finally, sequentially carrying out finishing dyeing, dehydration, transparence, wax dipping and embedding, slicing, sticking and unfolding, dewaxing, dyeing and sealing. The section obtained by the invention has the advantages of complete tissue, clear structure, strong operability and practicability, and is an effective method for observing the fruit thorn tumor anatomical structure.