摘要：【中文】本发明公开了与小麦株高主效QTL紧密连锁的分子标记及其应用。与小麦株高性状相关的分子标记有2个，1个是以小麦京411基因组DNA为模板，用SEQ ID NO:1和SEQ ID NO:2所示的引物对I进行PCR扩增得到的DNA片段，记为qPH‑5A‑ID2；另外1个也是以小麦京411基因组DNA为模板，用SEQ ID NO:3和SEQ ID NO:4所示的引物对II进行PCR扩增得到的DNA片段，记为qPH‑5A‑ID3。本发明提供的分子标记qPH‑5A‑ID2和qPH‑5A‑ID3，与qPH‑5A紧密连锁，能促进该位点在推广品种中转移应用，也有利于该位点与其它产量相关位点的聚合育种。 【EN】The invention discloses a molecular marker closely linked with a major QTL of wheat plant height and application thereof. The molecular markers related to the wheat plant height trait are 2, 1 is a DNA fragment obtained by carrying out PCR amplification on the I by using the primer shown in SEQ ID NO. 1 and SEQ ID NO. 2 and taking the wheat genome DNA 411 as a template, and is marked as qPH-5A-ID 2; another 1 DNA fragment was obtained by PCR amplification of II using the genomic DNA of Jing 411 from wheat as a template and primers shown in SEQ ID NO. 3 and SEQ ID NO. 4, and was designated qPH-5A-ID 3. The molecular markers qPH-5A-ID2 and qPH-5A-ID3 provided by the invention, andqPH‑5Athe close linkage can promote the transfer application of the locus in the popularization variety and is also beneficial to the polymerization breeding of the locus and other yield-related loci.

摘要：【中文】本发明公开了一种与小麦粒长主效QTL紧密连锁的分子标记及其应用，所述分子标记是以小麦京411基因组DNA为模板，用SEQ ID NO:1所示的上游引物和SEQ ID NO:2所示的下游引物进行PCR扩增得到的DNA片段，其核苷酸序列如SEQ ID NO:3所示。本发明提供的分子标记与qKl‑1BL紧密连锁，能够促进该位点在推广品种中转移应用，也有利于该位点与其它产量相关位点的聚合育种；本发明提供的分子标记为Indel标记，具有共显性，其引物对具有扩增稳定、检测快捷高效等优点，可以快速确定小麦资源中是否存在qKl‑1BL及存在型态，并预测小麦的粒长特性。 【EN】The invention discloses a molecular marker tightly linked with a major QTL of wheat grain length and application thereof, wherein the molecular marker is a DNA fragment obtained by carrying out PCR amplification by using wheat Jing 411 genome DNA as a template and using an upstream primer shown in SEQ ID NO. 1 and a downstream primer shown in SEQ ID NO. 2, and the nucleotide sequence of the DNA fragment is shown in SEQ ID NO. 3. The molecular marker provided by the invention andqKl‑1BLthe close linkage can promote the transfer application of the locus in the promoted variety and is also beneficial to the polymerization breeding of the locus and other yield-related loci; the molecular marker provided by the invention is an Indel marker, has codominance, has the advantages of stable amplification, quick and efficient detection and the like of a primer pair, and can quickly determine whether wheat resources exist or notqKl‑1BLAnd presence pattern, and predicting grain length characteristics of wheat.