摘要：【中文】本发明涉及一种原位分离检测核磁共振射频探头前端及其制备方法，带电粒子过滤管道、分离废液排出管道与样品检测管道的分叉分流配合，在竖直方向的磁场环境中，带电的顺磁性粒子受到沿径向向外的洛伦兹力作用，能够有效地在带电粒子过滤管道、分离废液排出管道与样品检测管道的交叉处准确分流，使分离出的带有顺磁性粒子的溶液由分离废液排出管道排出。本发明具有简单高效的磁性物质过滤分离结构，具有灵活的定制化样品管道设计，方便根据实验所需进行修改调整，有效提高检测信噪比，能够满足多种原位核磁共振检测的要求。本发明采用3D打印进行制备，可提高设计灵活度，并可大大提升效率，减少人工成本。 【EN】The invention relates to a front end of an in-situ separation detection nuclear magnetic resonance radio frequency probe and a preparation method thereof.A charged particle filtering pipeline, a separation waste liquid discharge pipeline and a sample detection pipeline are in branching and shunting fit, and charged paramagnetic particles are acted by a radially outward Lorentz force in a magnetic field environment in the vertical direction, so that the crossing parts of the charged particle filtering pipeline, the separation waste liquid discharge pipeline and the sample detection pipeline can be effectively and accurately shunted, and separated solution with paramagnetic particles is discharged by the separation waste liquid discharge pipeline. The invention has a simple and efficient magnetic substance filtering and separating structure, has flexible customized sample pipeline design, is convenient to modify and adjust according to experiment needs, effectively improves the detection signal-to-noise ratio, and can meet the requirements of various in-situ nuclear magnetic resonance detections. The invention adopts 3D printing for preparation, can improve the design flexibility, can greatly improve the efficiency and reduce the labor cost.

摘要：【中文】本发明公开了一种蛋白动态表达调控系统，属于生物工程技术领域。本发明通过分子生物学手段，利用生长期启动子和降解标签的特性设计构建出不需要借助人为控制和外源添加诱导剂的蛋白动态表达调控系统。引入该动态表达调控系统构建的生产莽草酸基因工程菌，能够实现动态调控莽草酸激酶的丰度变化，在无机盐培养基中，在不添加诱导剂和芳香族氨基酸条件下利用大肠杆菌生产莽草酸的能力，生产的莽草酸产量最高可达31g/L，是目前相同条件下已知报道的最高水平。 【EN】The invention discloses a protein dynamic expression regulation and control system, and belongs to the technical field of bioengineering. The invention designs and constructs a protein dynamic expression regulation and control system without human control and exogenous addition of an inducer by utilizing the characteristics of a growth phase promoter and a degradation label through a molecular biology means. The shikimic acid production genetic engineering bacteria constructed by introducing the dynamic expression regulation system can realize the dynamic regulation of the abundance change of shikimic acid kinase, and the shikimic acid yield can reach 31g/L at most under the condition of not adding an inducer and aromatic amino acid in an inorganic salt culture medium, and is the highest level known and reported under the same condition at present.

摘要：【中文】本发明公开了一种蛋白延时表达开关，通过组合使用生长期启动子P_rpsL和C末端降解标签SsrA，可以自动控制靶蛋白在细胞培养后期开启表达，且不需要借助人为控制和外源添加诱导剂。引入该蛋白延时表达开关构建的生产葡萄糖二酸的基因工程菌，能够控制目的蛋白肌醇‑1‑磷酸合成酶(INO1)仅当细胞密度达到一定程度后才开启表达，对于提高葡萄糖二酸合成效率具有重要价值，对于在微生物发酵生产葡萄糖二酸方面，也提供新的方法以及思路。 【EN】The invention discloses a protein time-delay expression switch, which uses a promoter P in a growth period in a combined way_rpsLAnd a C terminal degradation label SsrA, can automatically control the target protein to start expression in the later period of cell culture, and does not need to be assisted by artificial control and exogenous addition of an inducer. The gene engineering bacterium for producing the glucaric acid, which is constructed by introducing the protein time-delay expression switch, can control the expression of the target protein inositol-1-phosphate synthetase (INO1) to be started only when the cell density reaches a certain degree, has important value for improving the synthesis efficiency of the glucaric acid, and provides a new method and thought for producing the glucaric acid by microbial fermentation.

摘要：【中文】本发明提供了一种十六元大环内酯类化合物及其制备方法和应用。本发明所述化合物的结构式如下式I所示，该化合物I是由冰城链霉菌突变菌株Streptomyces bingchenggensis BC‑120‑4经培养和发酵，得到含有化合物I的发酵液，然后从发酵液中分离获得。本发明还涉及含有化合物I的农药组合物，以及化合物I在制备用于防治农作物病虫害的农药组合物中的用途。 【EN】The invention provides a hexadecanolide compound and a preparation method and application thereof. The structural formula of the compound is shown in the following formula I, and the compound I is obtained by culturing and fermenting Streptomyces bingchenggengnsis BC-120-4 serving as a Streptomyces paragesii mutant strain to obtain fermentation liquor containing the compound I and then separating the fermentation liquor. The invention also relates to a pesticide composition containing the compound I, and the application of the compound I in preparing a pesticide for preventing and controlling diseases and pests of cropsUse in pesticidal compositions.