摘要：【中文】本发明公开了一种自钻式土钉与加筋挡土墙联结系统及方法，包括边坡、加筋挡土墙和联结构件，边坡上钉入有若干个自钻式土钉，地基上构筑有加筋挡土墙，加筋挡土墙的加筋挡土墙构件通过联结构件与自钻式土钉连接固定；联结构件包括联结部件和纵向联结杆，联结部件由承压部件和环状握裹部组成，纵向联结杆紧密固定安装于环状握裹部中；加筋挡土墙构件包括加筋格网、水平支撑杆、联结绳索和夹持固定机构，加筋格网端部连接有水平支撑杆，水平支撑杆与纵向联结杆通过联结绳索连接。本发明在保证填方路基和陡立坡面交接处稳定性的同时降低加筋材因不均匀沉降带来的应力集中而致损的风险，提高了黄土地区高等级公路填方路基的建设质量和可靠性。 【EN】The invention discloses a system and a method for connecting self-drilling soil nails with a reinforced retaining wall, and the system comprises a slope, the reinforced retaining wall and connecting members, wherein a plurality of self-drilling soil nails are nailed into the slope, the reinforced retaining wall is constructed on a foundation, and the reinforced retaining wall member of the reinforced retaining wall is fixedly connected with the self-drilling soil nails through the connecting members; the connecting member comprises a connecting part and a longitudinal connecting rod, the connecting part consists of a pressure bearing part and an annular bonding part, and the longitudinal connecting rod is tightly and fixedly arranged in the annular bonding part; the reinforced retaining wall component comprises a reinforced grid, a horizontal support rod, a connecting rope and a clamping and fixing mechanism, wherein the end part of the reinforced grid is connected with the horizontal support rod, and the horizontal support rod is connected with the longitudinal connecting rod through the connecting rope. The method ensures the stability of the junction of the filling roadbed and the steep slope, reduces the risk of damage of the reinforced material due to stress concentration caused by uneven settlement, and improves the construction quality and reliability of the high-grade highway filling roadbed in the yellow land area.

摘要：【中文】本发明提供一种空气加速密封装置，在转轴的密封面外周侧固定有呈环形的密封体，密封体的轴向内侧为油槽，在密封体与转轴之间设有多个加速密封单元，所述加速密封单元固定在转轴上，随转轴同步旋转；所述加速密封单元、所述转轴以及所述密封体形成第一环槽腔，所述加速密封单元与所述密封体在靠近油槽一侧具有缝隙，使第一环槽腔与油槽相连通，所述加速密封单元在第一环槽腔内具有多个倾斜的扇叶，所述扇叶随着所述转轴旋转，使第一环槽腔内的空气向所述缝隙处集中。本发明的加速密封单元上的扇叶倾斜方向与转轴旋转方向相同，并且均匀分布在加速密封单元主体上。加速密封单元在转轴的旋转作用下，产生离心流体风压，使油槽内的油雾难以向外逸出。 【EN】The invention provides an air accelerating sealing device.A ring-shaped sealing body is fixed on the peripheral side of a sealing surface of a rotating shaft, the axial inner side of the sealing body is provided with an oil groove, a plurality of accelerating sealing units are arranged between the sealing body and the rotating shaft, and the accelerating sealing units are fixed on the rotating shaft and synchronously rotate along with the rotating shaft; with higher speed the sealing unit the pivot and the seal forms first ring groove chamber, with higher speed the sealing unit with the seal has the gap near oil groove one side, makes first ring groove chamber and oil groove be linked together, with higher speed the sealing unit has the flabellum of a plurality of slopes in first ring groove intracavity, the flabellum along with the pivot is rotatory, makes the air in first ring groove chamber to gap department concentrates. The inclined direction of the fan blades on the acceleration sealing unit is the same as the rotating direction of the rotating shaft, and the fan blades are uniformly distributed on the acceleration sealing unit main body. The acceleration sealing unit generates centrifugal fluid wind pressure under the rotation action of the rotating shaft, so that oil mist in the oil groove is difficult to escape outwards.

摘要：【中文】本发明提供一种多级弹性跟踪密封装置，包括转轴及安装在转轴外周侧的环形密封体，密封体的轴向内侧为油槽，密封体靠近转轴的环向内侧均布有偶数个楔形槽，每个楔形槽内安装有楔形自调密封环，所述楔形自调密封环由轴向弹性元件与径向弹性元件推动，与所述楔形槽以及转轴的外周面形成紧密接触。所述楔形自调密封环朝向转轴外周面设有间隙传送器，所述楔形自调密封环还固定连接有间隙调整杆，所述间隙调整杆与精确传动单元动力连接，所述精确传动单元以及所述间隙传送器均与一数显控制面板相连，如此形成自动间隙调整功能，可保证其与转轴的外周面接触时始终保持零间隙状态下运行，既安装方便，运行控制程序简单易操作，又能阻止油雾逸出。 【EN】The invention provides a multistage elastic tracking sealing device which comprises a rotating shaft and an annular sealing body arranged on the outer peripheral side of the rotating shaft, wherein the axial inner side of the sealing body is provided with an oil groove, even wedge-shaped grooves are uniformly distributed on the annular inner side of the sealing body close to the rotating shaft, a wedge-shaped self-adjusting sealing ring is arranged in each wedge-shaped groove, and is pushed by an axial elastic element and a radial elastic element to be in close contact with the wedge-shaped grooves and the outer peripheral surface of the rotating shaft. The wedge-shaped self-adjusting sealing ring is provided with a gap transmitter towards the peripheral surface of the rotating shaft, the wedge-shaped self-adjusting sealing ring is further fixedly connected with a gap adjusting rod, the gap adjusting rod is in power connection with the accurate transmission unit, the accurate transmission unit and the gap transmitter are both connected with a digital display control panel, so that an automatic gap adjusting function is formed, the wedge-shaped self-adjusting sealing ring can be guaranteed to always operate in a zero-gap state when contacting with the peripheral surface of the rotating shaft, the installation is convenient, the operation control program is simple and easy to operate, and oil mist can be prevented from escaping.

摘要：【中文】本发明公开了一种Atg12基因敲除细胞系构建方法及其应用，提供了用于敲除人结肠癌细胞Atg12基因的SgRNA、构建CRISPR‑Cas9载体，以及构建Atg12基因敲除细胞系的方法。本发明筛选获得的Atg12基因靶点，从多个靶点中优选出来的活性最好的靶点，高效靶向Atg12基因，而且对F2～F5代细胞敲除分析，敲除效率很高，位点均已被切割断，100％成功敲除；蛋白表达分析无Atg12蛋白，此方法可广泛应用于Atg12基因功能研究和药物研发等方面。 【EN】The invention discloses a construction method and application of an Atg12 gene knockout cell line, and provides a SgRNA for knocking out an Atg12 gene of a human colon cancer cell, a CRISPR-Cas9 vector and a method for constructing an Atg12 gene knockout cell line. The Atg12 gene target obtained by screening is a target with the best activity from a plurality of targets, and the Atg12 gene is efficiently targeted, and the knockout analysis of F2-F5 generation cells has high knockout efficiency, all sites are cut off, and 100% of successful knockout is realized; protein expression analysis has no Atg12 protein, and the method can be widely applied to the aspects of Atg12 gene function research, drug research and development and the like.

摘要：【中文】本发明公开了一种用于小断面隧道二衬的快速施工方法，在隧道贯通后进行洞内清理的同时，利用钢筋运输车、轮式自卸车将二衬钢筋运输、存放至洞内指定位置，隧道清理完成后，二衬由隧道两端向中间同时进行施工，先利用定制仰拱模板施工下部仰拱，然后利用多台门式拱墙模板台车跟进跳仓法施工上部拱墙，门式拱墙模板台车多节组成一套，节与节之间采用铰接连接，可适应曲线隧道施工。小断面隧道二衬快速施工方法单次施工节段长，工序衔接紧凑，可实现隧道二衬快速、高效施工，且整个施工过程机械化程度高，大大降低了劳动强度，施工模板均为整体定型化生产和使用，保证了工程质量，工期和经济效益显著。 【EN】The invention discloses a rapid construction method for a secondary lining of a small-section tunnel, which is characterized in that a reinforcing steel bar transport vehicle and a wheel type dump truck are utilized to transport and store the secondary lining reinforcing steel bars to a designated position in the tunnel while the tunnel is cleaned after the tunnel is communicated, after the tunnel is cleaned, the secondary lining is simultaneously constructed from two ends of the tunnel to the middle, a customized inverted arch template is utilized to construct a lower inverted arch, then a plurality of portal arch wall template trolleys are utilized to follow a skip method to construct an upper arch wall, the portal arch wall template trolleys form a set, and joints are connected in a hinged mode, so that the construction of a curve tunnel can be adapted. The two-lining rapid construction method for the small-section tunnel has the advantages that the single construction section is long, the procedure connection is compact, the two-lining rapid and efficient construction of the tunnel can be realized, the mechanization degree of the whole construction process is high, the labor intensity is greatly reduced, the construction templates are integrally formed, the engineering quality is ensured, and the construction period and the economic benefit are obvious.

摘要：【中文】本发明公开了一种用于小直径隧道二衬钢筋的转运及卸料方法，包括以下使用步骤：先将隧道区间划分为长度相同的节段，将单个节段所需各类钢筋在地面按顺序摆放至定制的型钢架子上，然后依次吊装上述型钢架子至竖井底部运输车组的平板车上，通过运输车组将该节段的钢筋运输至隧道内对应节段的卸料区域旁，最后用定制轮式起重机按照钢筋绑扎顺序的要求依次将本节段的钢筋从运输车上的型钢架子中吊出至卸料区，循环上述步骤，完成隧道区间二衬钢筋的洞内运输及堆放。本发明针对小直径隧道洞空间受限，常规卸料设备无法使用的情况，实现钢筋的快速转运、卸料，大幅提高钢筋的运输效率，且操作简单、机械化程度高，有效降低作业人员劳动强度。 【EN】The invention discloses a method for transferring and unloading a secondary lining steel bar in a small-diameter tunnel, which comprises the following steps: divide into the segment that length is the same with tunnel interval earlier, put the required all kinds of reinforcing bars of individual segment on customized shaped steel shelf according to the order on ground, then hoist above-mentioned shaped steel shelf to the flatbed of shaft bottom transport vechicle group in proper order, transport this segment's reinforcing bar to the tunnel in through the transport vechicle group by the regional side of unloading of corresponding segment, at last hang out this segment's reinforcing bar from the shaped steel shelf on the transport vechicle to the district of unloading in proper order with customized wheeled crane according to the requirement of reinforcement order, circulate above-mentioned step, accomplish the transportation in the hole of two lining reinforcing bars in the tunnel interval and stack. The steel bar conveying device disclosed by the invention is used for realizing the rapid transfer and unloading of the steel bars under the conditions that the space of a small-diameter tunnel hole is limited and the conventional unloading equipment cannot be used, greatly improving the conveying efficiency of the steel bars, being simple to operate and high in mechanization degree, and effectively reducing the labor intensity of operators.