摘要：【中文】本发明提供了一种粪便脱落细胞核酸保存试剂及其制备方法与应用，本发明的粪便脱落细胞核酸保存试剂包括非离子表面活性剂，阴离子表面活性剂，渗透压调节剂，细胞膜稳定剂，螯合剂，pH缓冲液和水，还含有除臭剂。本发明的保存试剂可以有效裂解粪便中的人体脱落细胞，且不含有硫氰酸胍和异硫氰酸胍等有毒成分，从而提供一种从粪便上清中直接进行粪便基因组提取和检测的方法，可以有效避免粪便杂质的干扰，检测前无需再次对粪便样品进行沉淀处理。 【EN】The invention provides a reagent for preserving nucleic acid of a fecal exfoliated cell, a preparation method and application thereof. The preservation reagent can effectively crack human exfoliative cells in the excrement, and does not contain toxic components such as guanidine thiocyanate, guanidine isothiocyanate and the like, so that the method for directly extracting and detecting the excrement genome from the excrement supernatant is provided, the interference of excrement impurities can be effectively avoided, and the excrement sample does not need to be subjected to precipitation treatment again before detection.