摘要：【中文】本发明属于荧光探针技术领域，具体涉及一种极低背景荧光的高灵敏次氯酸根荧光探针的制备方法。本发明提供了以8‑羟基久洛尼定‑9‑醛为起始原料，通过生成的香豆素343酰氯与2,4‑二硝基苯肼在三乙胺作用下合成该荧光探针的制备方法；该荧光探针是一种具有极低背景荧光、高灵敏的次氯酸根荧光探针，能选择性识别次氯酸根，且随着次氯酸根的浓度增加，其荧光强度在488nm逐渐增强；该探针具备灵敏、快速识别的功能；本发明制备的荧光探针可用于实际水样中次氯酸根含量的检测、活细胞外源性/内源性次氯酸根检测以及斑马鱼体内次氯酸根的检测。 【EN】The invention belongs to the technical field of fluorescent probes, and particularly relates to a preparation method of a highly sensitive hypochlorite fluorescent probe with extremely low background fluorescence. The invention provides a preparation method for synthesizing a fluorescent probe by using 8-hydroxy julolidine-9-aldehyde as an initial raw material through the generated coumarin 343 acyl chloride and 2, 4-dinitrophenylhydrazine under the action of triethylamine; the fluorescent probe is a hypochlorite fluorescent probe with extremely low background fluorescence and high sensitivity, can selectively identify hypochlorite, and the fluorescence intensity of the hypochlorite fluorescent probe is gradually enhanced at 488nm along with the increase of the concentration of the hypochlorite; the probe has the functions of sensitivity and quick identification; the fluorescent probe prepared by the invention can be used for detecting the hypochlorite content in an actual water sample, detecting exogenous/endogenous hypochlorite in living cells and detecting hypochlorite in a zebra fish body.

摘要：【中文】本发明属于有色金属冶炼技术领域，具体涉及一种铜或铜合金材料的制备方法及精炼用精炼剂。本发明的铜或铜合金材料精炼用精炼剂为由Cu元素、RE元素、M元素构成的合金；所述RE元素为稀土元素，所述M元素为碱金属元素或碱土金属元素。本发明的精炼剂能够与氧、磷、硫杂质生成密度较小的化合物，从而达到去除杂质的目的。采用本发明的精炼剂精炼后的铜或铜合金材料中的氧、磷以及硫的含量均为ppm级，并且制备成本较低。 【EN】The invention belongs to the technical field of non-ferrous metal smelting, and particularly relates to a preparation method of a copper or copper alloy material and a refining agent for refining. The refining agent for refining the copper or copper alloy material is an alloy consisting of a Cu element, a RE element and an M element; the RE element is a rare earth element, and the M element is an alkali metal element or an alkaline earth metal element. The refining agent can generate a compound with lower density with oxygen, phosphorus and sulfur impurities, thereby achieving the purpose of removing the impurities. The contents of oxygen, phosphorus and sulfur in the copper or copper alloy material refined by the refining agent are ppm level, and the preparation cost is low.