摘要：【中文】本发明提供了一种基于结合N‑乙酰半乳糖胺多肽的mRNA靶向分子及其制备方法，含有启动子、目的基因、特异性蛋白酶剪切序列、能结合N‑乙酰半乳糖胺的多肽GBD序列依次连接而成的DNA片段的质粒载体通过转录得到mRNA，在T4连接酶的作用下与DNA‑嘌呤霉素连接体相连接，通过蛋白翻译，并采用特异性蛋白酶进行剪切，得到mRNA‑嘌呤霉素‑GBD复合物，在N‑乙酰半乳糖胺转移酶作用下，与GBD蛋白序列结合形成mRNA‑嘌呤霉素‑GBD‑GalNAc复合物，从而对mRNA进行GalNAc修饰，在mRNA药物递送过程中，达到精准给药的目的，增加了mRNA药物分子的药效。 【EN】The invention provides an mRNA targeting molecule based on polypeptide combined with N-acetylgalactosamine and a preparation method thereof, wherein a plasmid vector containing a DNA fragment formed by sequentially connecting a promoter, a target gene, a specific protease cleavage sequence and a polypeptide GBD sequence capable of combining with N-acetylgalactosamine is transcribed to obtain mRNA, the mRNA is connected with a DNA-puromycin connector under the action of T4 ligase, the mRNA-puromycin-GBD compound is obtained through protein translation and cleavage by adopting specific protease, and the mRNA-puromycin-GBD compound is combined with the GBD protein sequence to form the mRNA-puromycin-GBD-GalNAc compound under the action of N-acetylgalactosamine transferase, so that the mRNA is subjected to GalNAc modification, the aim of accurate drug administration is fulfilled in the mRNA drug delivery process, and the drug effect of mRNA drug molecules is increased.

摘要：【中文】本发明公开一种规模化合成长链RNA的方法及其定点修饰的方法，所述规模化合成长链RNA的方法包括：设计RNA短片段和夹板DNA片段；连接；加帽；去夹板DNA片段等步骤。本发明技术方案通过化学法合成大量RNA短片段和不同的夹板DNA片段，然后通过生物法将不同RNA短片段连接起来以形成目的RNA长链。整个工艺操作简单、生产成本低，产物长链RNA的突变率低，并且单次反应可对多条RNA短片段进行组装，可高通量合成长链RNA，以满足长链RNA的规模化生产。此外，通过在RNA短片段上进行化学修饰，可实现对长链RNA的定点修饰。相较传统RNA化学修饰方法，本方法得到的RNA产物稳定性更高、免疫源性降低、翻译效率明显增强，对于RNA药物在临床上的应用有极其重要的作用。 【EN】The invention discloses a method for synthesizing long-chain RNA in a large scale and a fixed-point modification method thereof, wherein the method for synthesizing the long-chain RNA in the large scale comprises the following steps: designing RNA short fragments and splint DNA fragments; connecting; capping; and removing the splint DNA fragment and the like. The technical scheme of the invention synthesizes a large number of RNA short segments and different splint DNA segments by a chemical method, and then connects the different RNA short segments by a biological method to form a target RNA long chain. The whole process is simple to operate, low in production cost and low in mutation rate of long-chain RNA (ribonucleic acid) of a product, a plurality of short RNA segments can be assembled in a single reaction, and the long-chain RNA can be synthesized at high flux so as to meet the requirement of large-scale production of the long-chain RNA. In addition, by chemical modification on short segments of RNA, site-directed modification of long-chain RNA can be achieved. Compared with the traditional RNA chemical modification method, the RNA product obtained by the method has higher stability, reduced immunogenicity and obviously enhanced translation efficiency, and has extremely important effect on the clinical application of RNA medicaments.

摘要：【中文】本发明提供了一种环保清洗剂前体，其特征在于，由紫胶桐酸、聚乙二醇制备而成；所述紫胶桐酸和聚乙二醇重量份数比为：100：50‑100；所述环保清洗剂前体的制备方法为：将紫胶桐酸和聚乙二醇加入到反应釜内，加入催化剂；在氮气保护的情况下，于180～220℃的反应温度反应3～5小时；减压排除水分；即可。一种由前述环保清洗剂前体制备的环保清洗剂，还包括：如下原料制备而成：槐糖脂；富马酸二甲酯；异硬脂醇；失水山梨醇脂肪酸酯；太古油；消泡剂；去离子水。本发明制备的环保清洗剂前体是一种紫胶桐酸聚乙二醇酯，是良好的非离子型表面活性剂，由于所制备的酯分子结构中具有多个‑OH和多个EO基团，因此活性高，同时生物降解性好，对环境友好。 【EN】The invention provides an environment-friendly cleaning agent precursor which is characterized by being prepared from eleostearic acid and polyethylene glycol; the weight part ratio of the laccaic acid to the polyethylene glycol is as follows: 100: 50-100 parts of; the preparation method of the environment-friendly cleaning agent precursor comprises the following steps: adding lac eleostearic acid and polyethylene glycol into a reaction kettle, and adding a catalyst; reacting for 3-5 hours at the reaction temperature of 180-220 ℃ under the protection of nitrogen; reducing pressure to remove water; and (4) finishing. An environment-friendly cleaning agent prepared from the environment-friendly cleaning agent precursor further comprises: the material is prepared from the following raw materials: sophorolipid; dimethyl fumarate; isostearyl alcohol; sorbitan fatty acid esters; turkey red oil; defoaming agents; deionized water. The precursor of the environment-friendly cleaning agent prepared by the invention is a lac eleostearic acid polyethylene glycol ester which is a good nonionic surfactant, and the prepared ester has a molecular structure with a plurality of-OH and EO groups, so the environment-friendly cleaning agent has high activity, good biodegradability and environment friendliness.

摘要：【中文】本发明提供的一种润滑剂组合物，其特征在于,其特征在于,由紫胶桐酸、脂肪酸制备而成；所述紫胶桐酸和脂肪酸的摩尔比为1:1‑2。采采用本发明制备的润滑剂组合物,是一种紫胶桐酸脂肪酸酯，是良好的非离子型表面活性剂和润滑剂，润滑性能良好；同时生物降解性好，对环境友好。本发明还提供了用润滑剂组合物制备微量润滑剂，其特征在于，由以下重量百分比的组分制备而成：润滑剂组合物30‑40％；槐糖脂20‑30％；聚蔗糖脂肪酸酯10‑20％；蓖麻油磺酸钠5‑10％；异硬脂醇5‑10％；去离子水余量。本发明制备的微量润滑剂能满足金属加工的润滑冷却、极压抗磨和防锈要求；配合微量润滑装置使用，可节省润滑剂的使用量90％以上。 【EN】The invention provides a lubricant composition which is characterized by being prepared from laccaic acid and fatty acid; the molar ratio of the laccaic acid to the fatty acid is 1: 1-2. The lubricant composition prepared by the invention is a lac eleostearic acid fatty acid ester, is a good nonionic surfactant and a lubricant, and has good lubricating property; meanwhile, the biodegradability is good, and the environment is friendly. The invention also provides a trace lubricant prepared from the lubricant composition, which is characterized by being prepared from the following components in percentage by weight: 30-40% of a lubricant composition; 20-30% of sophorolipid; 10-20% of polysucrose fatty acid ester; 5-10% of sodium castor oil sulfonate; 5-10% of isostearyl alcohol; the balance of deionized water. The trace lubricant prepared by the invention can meet the requirements of lubrication cooling, extreme pressure wear resistance and rust prevention in metal processing; the lubricant is matched with a trace lubrication device for use, so that the use amount of the lubricant can be saved by more than 90%.

摘要：【中文】本发明属于陶瓷材料技术领域，公开了一种高Q值低温烧结锂镁钛系介质材料及其制备方法，其化学式为Li₂(Mg_1‑xZn_x)₃Ti(O_0.96F_0.08)₆，0.02≤x≤0.08；是将Li₂CO₃、MgO、TiO₂、LiF按比例配料；一次球磨后烘干、过筛，在一定温度下煅烧得到预烧粉末；预烧粉末二次球磨后烘干、过筛，进行造粒，过筛后压制成坯体；坯体在一定温度下烧结，保温后降至室温得到。本发明采用传统固相法，通过F^‑离子取代O^2‑离子降低锂镁钛陶瓷预烧温度和烧结温度的同时，通过Zn²⁺离子取代提高陶瓷的Q×f值，制备出具有高Q值的低温烧结的Li₂Mg₃TiO₆系微波介质陶瓷材料。 【EN】The invention belongs to the technical field of ceramic materials, and discloses a high-Q-value low-temperature sintered lithium-magnesium-titanium dielectric material and a preparation method thereof, wherein the chemical formula of the material is Li₂(Mg_1‑xZn_x)₃Ti(O_0.96F_0.08)₆X is more than or equal to 0.02 and less than or equal to 0.08; is prepared from Li₂CO₃、MgO、TiO₂Proportioning LiF according to a proportion; carrying out primary ball milling, drying, sieving, and calcining at a certain temperature to obtain pre-sintered powder; the pre-sintered powder is dried, sieved, granulated, sieved and pressed into a green body after secondary ball milling; sintering the blank at a certain temperature, and cooling to room temperature after heat preservation to obtain the product. The invention adopts the traditional solid phase method and adopts F^‑Ionic substitution of O^2‑When the pre-sintering temperature and the sintering temperature of the lithium-magnesium-titanium ceramic are reduced by ions, Zn passes through²⁺Ionic substitution to increase Q f value of ceramics and produce low temperature sintered Li with high Q value₂Mg₃TiO₆Is a microwave dielectric ceramic material.

摘要：【中文】本发明公开了一种处理现实生活中分类型数据的有效方法，包含以下步骤：步骤1，随机从含有n个样本的数据集X中选取k个初始点，k为数据集X的分类数目；步骤2，计算每个对象到k个初始点的距离，将对象分配到与其距离最小的初始点类中，得到k个簇；步骤3，计算每个对象到k个中心的隶属度，并用启发式更新算法更新k个簇的类中心，类中心表示为w_h；步骤4，重复步骤2、步骤3，直到类中心w_h不变为止。本发明的核心是基于分类型矩阵对象数据的MD fuzzy k‑modes聚类算法，大数据时代，利用MD fuzzy k‑modes算法对多条记录进行聚类，能更易发现顾客的消费偏好，从而做出更有针对性的推荐。 【EN】The invention discloses an effective method for processing classification data in real life, which comprises the following steps: step 1, randomly selecting k initial points from a data set X containing n samples, wherein k is the classification number of the data set X; step 2, calculating the distance between each object and k initial points, and distributing the objects to the initial point class with the minimum distance to the objects to obtain k clusters; step 3, calculating the membership degree of each object to k centers, and updating the class centers of the k clusters by using a heuristic updating algorithm, wherein the class centers are represented as w_h(ii) a Step 4, repeating the step 2 and the step 3 until the class center w_hUntil unchanged. The core of the invention is an MD fuzzy k-modes clustering algorithm based on classification type matrix object data, and in a big data era, a plurality of records are clustered by using the MD fuzzy k-modes algorithm, so that the customer's consumption can be found more easilyAnd the preference is paid, so that more targeted recommendations are made.

摘要：【中文】本发明公开了一种多功能医疗照明光源装置，包括承载管，所述承载管的底端活动连接有与其同轴的封板，所述封板为环状，所述封板的内侧螺纹连接有传动管，所述承载管内侧的底端固定连接有挤压机构。本发明涉及医用照明设备领域。该多功能医疗照明光源装置通过挤压机构实现了对膨胀机构膨胀大小的控制，调整对其内侧光源光线反射角度的改变，以达到控制光源光线焦距的目的，使提供良好且可控的观测效果，通过膨胀机构的膨胀效果，实现膨胀机构能够在物体的间隙，通孔内实现位置固定效果利用传动杆与承载管同轴错位的效果实现膨胀套的收束和展开，该方式能够方便光源进入各种狭小的窄道和间隙。这解决了照明光源装置聚光焦点调整不方便的问题。 【EN】The invention discloses a multifunctional medical lighting source device which comprises a bearing tube, wherein the bottom end of the bearing tube is movably connected with a sealing plate coaxial with the bearing tube, the sealing plate is annular, the inner side of the sealing plate is in threaded connection with a transmission tube, and the bottom end of the inner side of the bearing tube is fixedly connected with an extrusion mechanism. The invention relates to the field of medical lighting equipment. This multi-functional medical lighting light source device has realized the control to the inflation size of expansion mechanism through extrusion mechanism, the adjustment is to its inboard light reflection angle's change, in order to reach the purpose of control light source light focus, make to provide good and controllable observation effect, inflation effect through expansion mechanism, realize that expansion mechanism can be in the clearance of object, realize in the through-hole that the fixed effect in position utilizes the effect of transfer line and the coaxial dislocation of bearing pipe to realize the receipts of inflation cover and expand, this mode can make things convenient for the light source to get into various narrow and small lanes and clearance. The problem that the condensation focus of the illumination light source device is inconvenient to adjust is solved.

摘要：【中文】本发明公开了一种可放松的带音乐播放器的血透患者用病床，涉及医疗技术领域，其包括支撑板，所述支撑板上开设有通孔，且通孔前后两侧壁上均开设有滑动槽。该可放松的带音乐播放器的血透患者用病床，通过病人面部朝上躺在软垫上，之后使病人进行透析治疗，在进行透析治疗时，病人可以根据控制盒控制音乐箱播放音乐，进而对自己的精神进行放松，同时也可以通过控制控制电机转动，继而利用齿轮一、齿轮三、盘卷盘二、连接绳一、T形滑槽、导向轮、T形滑块、连接绳二、盘卷盘一、齿轮四、齿轮二以及支撑杆实现T形滑块在T形滑槽内左右往复运动，最终解决了血液透析患者血液透析时容易紧张以及血压不足，继而导致血液透析失败的问题。 【EN】The invention discloses a relaxing sickbed with a music player for a hemodialysis patient, which relates to the technical field of medical treatment. This sick bed is used to patient is passed through to blood of taking music player that can relax, lie on the soft pad up through patient's face, later make patient carry out the dialysis treatment, when carrying out the dialysis treatment, patient can control the music box according to the control box and play the music, and then relax the spirit of oneself, also can rotate through control motor simultaneously, then utilize gear one, gear three, dish reel two, connect rope one, T shape spout, the leading wheel, T shape slider, connect rope two, dish reel one, gear four, gear two and bracing piece realize that T shape slider is reciprocating motion about in T shape spout, easy nervous and blood pressure is not enough when finally having solved hemodialysis patient hemodialysis, then lead to the problem of hemodialysis failure.

摘要：【中文】本发明公开了一种船用保形增稳推进装置，属于船用侧推设备技术领域，所述船用保形增稳推进装置设置在船体底部，该装置包括伸缩杆、设置在伸缩杆上的保形板、设置在保形板内部驱动电机和设置在驱动电机上的驱动螺旋桨；所述船体底部设有容纳腔，所述伸缩杆的一端与容纳腔底部连接，另一端与保形板连接，伸缩杆收缩到最短时，保形板与伸缩杆全部收收缩到容纳腔内部；所述保形板与船体的纵向轴线平行，保形板上还有通孔，通孔的轴线与船体的纵向轴线垂直，驱动电机和设置在驱动电机输出轴上的驱动螺旋桨设置在通孔内。通过推进装置的收缩到达保形的功能，通过推进装置的的伸出达到增加船体稳定性的作用。 【EN】The invention discloses a marine conformal stability-increasing propelling device, which belongs to the technical field of marine side-pushing equipment and is arranged at the bottom of a ship body, wherein the marine conformal stability-increasing propelling device comprises a telescopic rod, a shape-maintaining plate arranged on the telescopic rod, a driving motor arranged in the shape-maintaining plate and a driving propeller arranged on the driving motor; the bottom of the ship body is provided with an accommodating cavity, one end of the telescopic rod is connected with the bottom of the accommodating cavity, the other end of the telescopic rod is connected with the shape-preserving plate, and when the telescopic rod is contracted to the shortest length, the shape-preserving plate and the telescopic rod are completely contracted into the accommodating cavity; the shape-preserving plate is parallel to the longitudinal axis of the ship body, the shape-preserving plate is also provided with a through hole, the axis of the through hole is vertical to the longitudinal axis of the ship body, and the driving motor and the driving propeller arranged on the output shaft of the driving motor are arranged in the through hole. The function of shape keeping is achieved through the contraction of the propelling device, and the function of increasing the stability of the ship body is achieved through the extension of the propelling device.

摘要：【中文】本发明公开了一种船轴提升装置，包括上支架、下支架、提升件；所述提升件设于所述上支架和所述下支架之间，用于提升上支架；所述上支架表面设有滑动件，所述船轴固定于滑动件内；所述下支架内部设有调节组件；所述调节组件与提升件配合连接，可控制提升件的高度。解决了船轴在安装过程中，都是通过吊装机构将船轴吊起来，吊起的高度只能够大致满足安装的需要，会存在一定的偏差的问题。 【EN】The invention discloses a ship shaft lifting device, which comprises an upper bracket, a lower bracket and a lifting piece, wherein the upper bracket is fixedly connected with the lower bracket; the lifting piece is arranged between the upper bracket and the lower bracket and used for lifting the upper bracket; the surface of the upper bracket is provided with a sliding part, and the ship shaft is fixed in the sliding part; an adjusting component is arranged in the lower bracket; the adjusting component is connected with the lifting piece in a matching way, and the height of the lifting piece can be controlled. The problem of the ship axle in the installation, all hoist the ship axle through hoisting mechanism, the height of hoisting only can roughly satisfy the needs of installation, can have certain deviation is solved.