摘要：【中文】本发明公开了一种羟苯酯类凝胶剂，涉及医药技术领域。该羟苯酯类凝胶剂含有卡波姆，有利于羟苯酯类抑菌剂在凝胶剂中均匀分散，促使抑菌剂更好的发挥药效；柠檬酸有助于促进卡波姆溶解分散，形成均匀的悬浮液；功能性低聚糖成分对乳酸杆菌以及上皮细胞有促进增殖、生长的作用，有助于加快恢复受损部位；酸度调节剂可调节凝胶剂的pH值，确保对羟苯酯类凝胶剂不排斥、无刺激，提高患者的使用体验；本技术方案在各组分的共同作用下可起到协同增效的作用，使得羟苯酯类凝胶剂中的杀菌物质稳定，具有良好的抑菌杀菌效果和生物相容性、无刺激以及更好使用体验，可以更好地发挥抑菌杀菌作用。 【EN】The invention discloses a hydroxyphenyl ester gel, and relates to the technical field of medicines. The hydroxyphenyl ester gel contains carbomer, which is beneficial to uniformly dispersing hydroxyphenyl ester bacteriostatic agent in the gel and promoting the bacteriostatic agent to better exert the drug effect; citric acid helps to promote the dissolution and dispersion of carbomer to form a uniform suspension; the functional oligosaccharide component has proliferation and growth promoting effects on lactobacillus and epithelial cells, and helps to accelerate recovery of damaged parts; the acidity regulator can adjust the pH value of the gel, ensure that the parabens gel is not rejected and irritated, and improve the use experience of patients; the technical scheme can play a role in synergy under the combined action of all components, so that the sterilizing substance in the hydroxyphenyl ester gel is stable, has good antibacterial and bactericidal effects and biocompatibility, is free of irritation and better in use experience, and can better play a role in bacteriostasis and sterilization.

摘要：【中文】本发明公开转基因大豆GTS‑40‑3‑2品系EFIRM检测探针及其应用。所述检测探针包括SEQ ID NO.1‑4的核苷酸序列。本发明针对转基因大豆GTS‑40‑3‑2品系特异性基因，自主设计了EFIRM法检测转基因大豆GTS‑40‑3‑2品系的特异性捕获探针和杂交探针，采用EFIRM技术对食品及饲料中转基因大豆GTS‑40‑3‑2品系成分进行定性检测。本发明对提高食品和饲料中转基因大豆GTS‑40‑3‑2品系的检测效率及灵敏度，降低检测成本，检测进出口和国内市场上相关转基因产品，进行食品安全转基因监测和提高转基因检测技术具有重要意义，具有广阔发展前景。 【EN】The invention discloses a transgenic soybean GTS-40-3-2 strain EFIRM detection probe and application thereof. The detection probe comprises a nucleotide sequence of SEQ ID NO. 1-4. The invention designs a specific capture probe and a hybridization probe for detecting the transgenic soybean GTS-40-3-2 strain by an EFIRM method aiming at the specific gene of the transgenic soybean GTS-40-3-2 strain, and adopts the EFIRM technology to carry out qualitative detection on the components of the transgenic soybean GTS-40-3-2 strain in food and feed. The invention has important significance for improving the detection efficiency and sensitivity of the transgenic soybean GTS-40-3-2 strain in food and feed, reducing the detection cost, detecting related transgenic products in import and export and domestic markets, carrying out food safety transgenic monitoring and improving transgenic detection technology, and has wide development prospect.

摘要：【中文】本发明公开了一种羧基乳胶微球与抗体的偶联方法，涉及医学检测技术领域。该方法通过对羧基化乳胶微球进行预处理、对羧基化乳胶微球进行活化形成活化中间体，最后再与抗体偶联的过程，有效地提高羧基乳胶微球单分散性、乳胶微球活化中间体的浓度以及羧基乳胶微球与抗体的偶联效率，避免乳胶微球发生自凝，维持乳胶微球的活化状态从而提高微球与抗体的有效结合率。 【EN】The invention discloses a coupling method of carboxyl latex microspheres and an antibody, and relates to the technical field of medical detection. The method effectively improves the monodispersity of the carboxyl latex microspheres, the concentration of the activated intermediates of the latex microspheres and the coupling efficiency of the carboxyl latex microspheres and the antibodies through the processes of pretreating the carboxyl latex microspheres, activating the carboxyl latex microspheres to form activated intermediates, and finally coupling the activated intermediates with the antibodies, avoids the self-coagulation of the latex microspheres, and maintains the activated state of the latex microspheres so as to improve the effective binding rate of the microspheres and the antibodies.

摘要：【中文】本发明提供了一种用于检测金黄色葡萄球菌的引物组合和试剂盒，属于生物检测技术领域，引物组合包括内部引物组和外部引物组；内部引物组包括上游内部引物和下游内部引物，上游内部引物的核苷酸序列如SEQ ID No.3、7、12或17所示，下游内部引物的核苷酸序列如SEQ ID No.4、8、13或18所述；外部引物组包括上游外部引物和下游外部引物，上游外部引物的核苷酸序列如SEQ ID No.1、5、10或15所示，下游外部引物的核苷酸序列如SEQ ID No.2、6、11或16所示。本发明的引物组合对金黄色葡萄球菌具有高度的特异性，结合等温扩增方法能够缩短检测的时间，仅需45min就能够得出检测结果。 【EN】The invention provides a primer combination and a kit for detecting staphylococcus aureus, belonging to the technical field of biological detection, wherein the primer combination comprises an inner primer group and an outer primer group; the inner primer group comprises an upstream inner primer and a downstream inner primer, the nucleotide sequence of the upstream inner primer is shown as SEQ ID No.3, 7, 12 or 17, and the nucleotide sequence of the downstream inner primer is shown as SEQ ID No.4, 8, 13 or 18; the external primer group comprises an upstream external primer and a downstream external primer, wherein the nucleotide sequence of the upstream external primer is shown as SEQ ID No.1, 5, 10 or 15, and the nucleotide sequence of the downstream external primer is shown as SEQ ID No.2, 6, 11 or 16. The primer combination disclosed by the invention has high specificity to staphylococcus aureus, and can shorten the detection time by combining an isothermal amplification method, and the detection result can be obtained only in 45 min.