摘要：【中文】本发明涉及一种表达细粒棘球蚴EG95蛋白的重组狂犬病病毒及其构建方法与应用。本发明通过反向遗传技术将人工优化的细粒棘球蚴编码EG95蛋白的基因序列插入到狂犬病病毒基因组中，获得了免疫原性好、毒力弱、复制滴度高的重组狂犬病病毒，重组狂犬病病毒在NA细胞中的滴度达到3.2×10⁸FFU/mL，与亲本狂犬病病毒SAD株的生长滴度相近，重组狂犬病病毒还可诱导小鼠产生狂犬病病毒的中和抗体和EG95特异性保护抗体，且安全性高，可为狂犬病病毒和细粒棘球蚴的感染提供有效的保护，可用于制备预防狂犬病和囊型包虫病二联疫苗。 【EN】The invention relates to a recombinant rabies virus for expressing echinococcus granulosus EG95 protein and a construction method and application thereof. The invention relates to a gene sequence of an artificially optimized echinococcus granulosus EG95 protein coded by the echinococcus granulosus by a reverse genetic technologyThe recombinant rabies virus with good immunogenicity, weak virulence and high replication titer is obtained by inserting the recombinant rabies virus into a rabies virus genome, and the titer of the recombinant rabies virus in NA cells reaches 3.2 multiplied by 10⁸FFU/mL is similar to the growth titer of a parental rabies virus SAD strain, the recombinant rabies virus can also induce a mouse to generate a neutralizing antibody of the rabies virus and an EG95 specific protective antibody, has high safety, can provide effective protection for the infection of the rabies virus and echinococcosis granulosa, and can be used for preparing a bivalent vaccine for preventing rabies and cysticercosis.

摘要：【中文】本发明提供了一种农业辅助生产设备，尤其涉及用于烤烟生产的保水剂施用装置，该装置包括机架、料箱、开沟器、覆土装置、滚轮、推手、固定块及螺杆；所述左右机架通过固定块、螺杆连接，左右两个机架上均焊接有弹性卡固件、螺纹连接管，开沟器、推手分别与机架上对应的螺纹连接管螺纹连接，料箱与机架上的弹性卡固件活动连接，料箱顶部及底端出口位置均装有可开、关的盖子，覆土装置与机架焊接，机架均装有滚轮。本发明结构简单，小巧便捷，操作方便，采用模块化设计理念可根据实际需求灵活调整作业间距，适用于年降雨较少、海拔相对较高的山地烟区和一些小面积土壤贫瘠的植烟区。 【EN】The invention provides agricultural auxiliary production equipment, in particular to a water-retaining agent applying device for flue-cured tobacco production, which comprises a rack, a material box, a furrow opener, a soil covering device, rollers, a pushing handle, a fixed block and a screw rod, wherein the rack is provided with a plurality of grooves; the machine frame passes through fixed block, screw rod connection about, all welds elastic clamping spare, threaded connection pipe on two frames about, furrow opener, pushing hands respectively with the threaded connection pipe threaded connection who corresponds in the frame, the workbin with the elastic clamping spare swing joint in the frame, the lid that can open and shut is all equipped with to workbin top and bottom exit position, earthing device and frame welding, the gyro wheel is all equipped with to the frame. The tobacco planting device is simple in structure, small, exquisite, convenient and fast, convenient to operate, capable of flexibly adjusting the operation space according to actual requirements by adopting a modular design concept, and suitable for mountain tobacco areas with less rainfall and relatively high altitude and certain tobacco planting areas with poor soil in small area.

摘要：【中文】本发明提供了一种利用铜、铅锌冶炼废水污泥制备的硫铝酸盐水泥及其制备方法，主要由以下质量份的原料制备而成：经过干燥处理的有色金属冶炼废弃物2～15份、石灰石30～60份、铝矾土15～30和校正原料3～10份。有色金属冶炼废弃物包括铜火法冶炼过程中污酸处理系统的中和渣、锌和铅冶炼过程中通过污酸系统后形成的废水的处理污泥中的一种。本发明所提供的硫铝酸盐水泥，由既定比例的石灰石、铝矾土矾土、和以石膏为主要原料的有色金属冶炼废弃物制备得到，具有低碱度、高早强、微膨胀、耐侵蚀和抗冻性好的优点，为铜铅锌冶炼行业所产生的危险废物的处理与资源化利用提供了一种合理的解决方案，有良好的经济、环境和社会效益。 【EN】The invention provides sulphoaluminate cement prepared from copper, lead and zinc smelting wastewater sludge and a preparation method thereof, and the sulphoaluminate cement is mainly prepared from the following raw materials in parts by mass: 2-15 parts of non-ferrous metal smelting waste subjected to drying treatment, 30-60 parts of limestone, 15-30 parts of bauxite and 3-10 parts of correction raw materials. The non-ferrous metal smelting waste comprises neutralization slag of a waste acid treatment system in the copper pyrometallurgical process and one of treatment sludge of waste water formed after the waste acid treatment system passes through the zinc and lead metallurgical process. The sulphoaluminate cement provided by the invention is prepared from limestone, bauxite and non-ferrous metal smelting wastes which take gypsum as main raw materials according to a predetermined proportion, has the advantages of low alkalinity, high early strength, micro-expansion, corrosion resistance and good freezing resistance, provides a reasonable solution for the treatment and resource utilization of dangerous wastes generated in the copper-lead-zinc smelting industry, and has good economic, environmental and social benefits.