摘要：【中文】本发明“Nitazoxanide在抑制犬细小病毒中的应用”,涉及已知药物的新用途。数据证明，小分子药物Nitazoxanide对CPV复制具有非常卓越的抑制作用，能够抑制不同基因型病毒VP2蛋白的表达。 【EN】The invention discloses application of Nitazoxanide in inhibiting canine parvovirus, and relates to new application of a known medicament. The data prove that the small molecule drug Nitazoxanide has excellent inhibition effect on CPV replication and can inhibit the expression of VP2 protein of different genotypes.

摘要：【中文】本发明提供了一种猪胸膜肺炎放线杆菌感受态细胞的制备及转化方法，将胸膜肺炎放线杆菌在TSB培养基中震荡培养至OD600为0.3～0.6，离心收集菌体，并采用改良氯化铷法制备感受态细胞，将最终制备的感受态细胞中加入终浓度为7％的DMSO，分装到无菌EP管，‑80℃保存；将感受态细胞与待转化质粒DNA混匀后，经冰浴、热击、复苏。本发明的方法操作简单、转化效率高、为胸膜肺炎放线杆菌的遗传改造提供了新的技术方法。 【EN】The invention provides a preparation and transformation method of porcine actinobacillus pleuropneumoniae competent cells, which comprises the steps of shake-culturing actinobacillus pleuropneumoniae in a TSB culture medium until OD600 is 0.3-0.6, centrifugally collecting thalli, preparing the competent cells by adopting an improved rubidium chloride method, adding DMSO with the final concentration of 7% into the finally prepared competent cells, subpackaging the competent cells in a sterile EP tube, and storing at-80 ℃; and (3) uniformly mixing the competent cells and the plasmid DNA to be transformed, and then carrying out ice bath, heat shock and recovery. The method disclosed by the invention is simple to operate and high in transformation efficiency, and provides a new technical method for genetic modification of actinobacillus pleuropneumoniae.

摘要：【中文】本公开提供了一种基于光纤单元的焦面板非平面阵列孔均分制造方法，包括：布置非平面阵列多孔；计算平面上各点的坐标值；通过可变焦点投影法计算平面上的各点在曲面上投影后的坐标值；比较上相邻两点间的间距；计算非平面阵列孔相邻各点间距最大误差；多次迭代优化误差，生成非平面阵列孔中心点的三维坐标。本公开能够制造具有高的位置精度安装光纤定位单元非平面阵列孔位，安装光纤单元后能够提高望远镜光学焦面上光谱接收能量效率，实现光纤在焦面上无盲区扫描覆盖。 【EN】The present disclosure provides a method for uniformly dividing and manufacturing a non-planar array hole of a focal plane based on an optical fiber unit, which comprises: arranging a non-planar array of wells; calculating coordinate values of each point on the plane; calculating coordinate values of each point on the plane after projection on the curved surface by a variable focus projection method; comparing the distance between the two adjacent points; calculating the maximum error of the distance between adjacent points of the non-planar array holes; and (5) iteratively optimizing the error for multiple times to generate a three-dimensional coordinate of the central point of the non-planar array hole. The non-planar array hole site with high position precision for installing the optical fiber positioning unit can be manufactured, the spectrum receiving energy efficiency on the optical focal plane of the telescope can be improved after the optical fiber unit is installed, and the scanning coverage of the optical fiber on the focal plane without blind areas is realized.